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首页> 外文期刊>Clinical and diagnostic laboratory immunology >CD4-Positive and CD8-Positive Cytotoxic T Lymphocytes Contribute to Human Papillomavirus Type 16 E6 and E7 Responses
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CD4-Positive and CD8-Positive Cytotoxic T Lymphocytes Contribute to Human Papillomavirus Type 16 E6 and E7 Responses

机译：CD4阳性和CD8阳性的细胞毒性T淋巴细胞有助于人类乳头瘤病毒16型E6和E7反应。

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摘要

Cytotoxic T-lymphocyte (CTL) responses to E6 and E7 were previously shown to be more commonly detectable in human papillomavirus type 16 (HPV-16)-positive women without squamous intraepithelial neoplasia (SIL) than in HPV-16-positive women with SIL (M. Nakagawa, D. P. Stites, S. Farhat, J. R. Sisler, B. Moss, F. Kong, A. B. Moscicki, and J. M. Palefsky, J. Infect. Dis. 175:927–931, 1997). The objective of this study was to characterize the phenotype(s) of the effector cell population responsible for HPV-16 E6- and E7-specific cytotoxic responses. Peripheral blood mononuclear cells were stimulated with HPV-16 E6 or E7 fusion protein. Cells from an autologous B-lymphoblastoid cell line, infected with vaccinia virus expressing E6 or E7, served as target cells. The effector cells were characterized by using natural-killer-cell removal, antibody blocking, and T-cell subset separation. Our results suggest that both CD4 and CD8 T lymphocytes contribute to HPV-16 E6- and E7-specific CTL responses although their relative contributions vary from individual to individual. On the other hand, natural killer cells in the effector cell population contribute to background activities but not to HPV-specific responses in this assay system.
机译：先前显示，在没有鳞状上皮内瘤样变（SIL）的人乳头瘤病毒16型（HPV-16）阳性女性中，对E6和E7的细胞毒性T淋巴细胞（CTL）反应比在SIL的HPV-16阳性女性中更常见（M. Nakagawa，DP Stites，S。Farhat，JR Sisler，B。Moss，F。Kong，AB Moscicki和JM Palefsky，J。Infect。Dis。175：927-931，1997年）。这项研究的目的是表征负责HPV-16 E6-和E7特异性细胞毒性反应的效应细胞群的表型。 HPV-16 E6或E7融合蛋白刺激外周血单核细胞。来自自体B淋巴母细胞系的细胞被表达E6或E7的牛痘病毒感染后用作靶细胞。通过使用自然杀伤细胞去除，抗体阻断和T细胞亚群分离来表征效应细胞。我们的结果表明，CD4和CD8 T淋巴细胞均对HPV-16 E6-和E7特异性CTL反应有贡献，尽管它们的相对贡献因人而异。另一方面，效应细胞群中的自然杀伤细胞有助于本底活性，但在该测定系统中无助于HPV特异性应答。

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