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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Comparison of the Frequencies and Levels of Human Immunodeficiency Virus Type 1 Markers in Specimens from Chronically Infected Human T-Lymphocyte Cultures and from Patients
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Comparison of the Frequencies and Levels of Human Immunodeficiency Virus Type 1 Markers in Specimens from Chronically Infected Human T-Lymphocyte Cultures and from Patients

机译：慢性感染人T淋巴细胞培养物和患者标本中人类免疫缺陷病毒1型标记物的频率和水平的比较

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Together with CD4+-cell counts as an indicator of immune function, the use of human immunodeficiency virus type 1 (HIV-1) RNA levels as a direct marker of viral load has gained widespread attention for evaluation of patient clinical status. Results obtained with other HIV-1 markers for this purpose are often inconsistent. This study examined the relationship between various HIV-1 markers by using clinical specimens (plasma) from HIV-1-infected individuals at different stages of disease progression and supernatant fluid from four human T-lymphocyte cell lines chronically infected with HIV-1. Cell culture specimens were collected periodically over 7 days and were tested for HIV-1 RNA levels with a nucleic acid amplification assay, for p24 with an enzyme-linked immunosorbent assay, and for reverse transcriptase activity by isotope uptake. An increase in the level of each marker was observed over the 7-day period with each of the four HIV-1 strains tested (LAV1, HTLV-IIIB, MN, and ARV2); with these specimens, the frequency of detection for each marker was 100%. In the clinical specimens, HIV-1 RNA was detected more often (143 of 183 specimens [78%]) than was p24 (87 of 183 [48%]); little correlation between the levels of the two markers was seen. In these clinical specimens evaluated, CD4+-cell counts were better correlated with the frequency and levels of HIV-1 RNA than with p24. In specimens (n = 38) collected serially from six HIV-1-infected subjects, HIV-1 RNA was detected more often (33 of 38 [85%]) than p24 (23 of 38 [59%]). When reported by the assays used, the levels of both HIV-1 markers fluctuated over time for each of the subjects. Although the markers correlated in the in vitro systems studied, the observed differences in the correlation of levels and frequencies of HIV-1 markers in vivo indicate that p24 has less clinical utility than does viral load testing when used in conjunction with CD4+-cell counts as a measure of immune system functioning.
机译：与CD4 + -细胞计数一起作为免疫功能的指标，使用人类1型免疫缺陷病毒（HIV-1）RNA水平作为病毒载量的直接标志物已受到广泛关注，以评估病人的临床状况。为此目的，使用其他HIV-1标记获得的结果通常不一致。这项研究通过使用来自处于疾病进展不同阶段的HIV-1感染者的临床标本（血浆）和来自四个长期感染HIV-1的人类T淋巴细胞系的上清液，检查了各种HIV-1标记之间的关系。在7天之内定期收集细胞培养标本，并通过核酸扩增测定法测试HIV-1 RNA水平，通过酶联免疫吸附测定法测试p24的水平，并通过同位素摄取来测试逆转录酶的活性。在测试的4种HIV-1菌株（LAV1，HTLV-IIIB，MN和ARV2）中，每7天观察到的每种标记物的水平都增加了。对于这些标本，每个标记的检测频率为100％。在临床标本中，检测到HIV-1 RNA的频率更高（183个标本中的143个[78％]）比p24（183个标本中的87个[48％]）检测的频率更高。两种标记物的水平之间几乎没有相关性。在这些评估的临床样本中，CD4 + -细胞计数与HIV-1 RNA的频率和水平的相关性高于与p24的相关性。在从六个感染HIV-1的受试者中连续收集的标本（ n = 38）中，检测到HIV-1 RNA的频率（在38个样本中占33个，占85％[85％]）比在p24样本中检测到38个样本中占23个[59] ％]）。当通过使用的检测方法报告时，每个受试者的两种HIV-1标记物的水平都会随时间波动。尽管标记物在研究的体外系统中具有相关性，但观察到的体内HIV-1标记物水平和频率的相关性差异表明，与CD4 +结合使用时，p24的临床效用低于病毒载量测试-细胞计数是衡量免疫系统功能的指标。

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