Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients

Lucio Vera-Cabrera; Adrian Rendon; Manuel Diaz-Rodriguez; Vera Handzel; Adalbert Laszlo

首页> 外文期刊>Clinical and diagnostic laboratory immunology >Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients

【24h】

Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients

机译：点印迹法检测结核病患者抗二乙酰海藻糖抗体

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A simple dot blot test with diacyltrehalose (DAT) as the antigen was developed to detect anti-DAT antibodies in tuberculous patients. To enhance antigen-antibody reaction detection, rabbit serum raised against human immunoglobulins was used prior to incubation with a protein A-colloidal gold complex. With the dot blot system, it was possible to obtain a sensitivity similar to that of enzyme-linked immunosorbent assay (ELISA) and a specificity of 97.14%, versus a specificity of 94.29% by the ELISA. We conclude that this simple and fast assay could be used in places where ELISA equipment is not easy available and that it might also be applicable with otherMycobacterium tuberculosis immunogenic antigens.

机译：开发了一种简单的以二乙酰海藻糖（DAT）作为抗原的斑点印迹测试来检测结核病患者中的抗DAT抗体。为了增强抗原-抗体反应检测，在与蛋白A-胶体金复合物孵育之前，先使用针对人免疫球蛋白的兔血清。使用斑点印迹系统，可以获得与酶联免疫吸附测定（ELISA）相似的灵敏度，特异性为97.14％，而ELISA的特异性为94.29％。我们得出的结论是，这种简单，快速的测定方法可用于不易获得ELISA设备的地方，也可能适用于其他结核分枝杆菌免疫原性抗原。 展开▼

著录项

来源
《Clinical and diagnostic laboratory immunology》 |1999年第5期|共4页

作者
Lucio Vera-Cabrera; Adrian Rendon; Manuel Diaz-Rodriguez; Vera Handzel; Adalbert Laszlo;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类诊断学;

关键词

相似文献

外文文献

中文文献

专利

1. Detection of mutation in embB gene of Mycobacterium tuberculosis from clinical isolates of tuberculous patients in China by means of reverse-dot blot hybridization [J] . XUE QIONG WU, YANG LU, JIAN QIN LIANG, 中华微生物学和免疫学杂志（英文版） . 2006,第001期

机译：反向斑点杂交技术检测中国结核病患者临床分离株结核分枝杆菌emB基因突变

2. An enhanced immuno-dot blot assay for the detection of white spot syndrome virus in shrimp using antibody conjugated gold nanoparticles probe. [J] . Chinnasamy Thiruppathiraja, Subramani Kumar, Vidhyapriya Murugan, Aquaculture . 2011,第3a4期

机译：一种增强的免疫斑点印迹测定法，使用抗体偶联的金纳米颗粒探针检测虾中的白斑综合症病毒。

3. Comparison of direct binding polymerase chain reaction with recombinant coat protein antibody based dot-blot immunobinding assay and immunocapture-reverse transcription-polymerase chain reaction for the detection of sugarcane streak mosaic virus caus [J] . Hema M, Savithri HS, Sreenivasulu P Current Science: A Fortnightly Journal of Research . 2003,第12期

机译：直接结合聚合酶链反应与基于重组外壳蛋白抗体的斑点印迹免疫结合法和免疫捕获逆转录聚合酶链反应用于检测甘蔗花叶病毒病因的比较

4. Quantum dot-based western blot for sensitive detection of pig serum antibody to actinobacillus pleuropneumoniae [C] . Ana Cismileanu, Cornelia Sima, Constantin Grigoriu ROMOPTO 2006: Eight Conference on Optics; Proceedings of SPIE-The International Society for Optical Engineering; vol.6785 . 2006

机译：基于量子点的蛋白质印迹用于灵敏检测猪胸膜肺炎放线杆菌的猪血清抗体

5. Development of DNA-tagged liposome biosensing devices and enzyme-linked dot blot assay in combination with nucleic acid sequence-based amplification for rapid detection of viable shiga toxin-producing Escherichia coli. [D] . Kao, Mou-Chieh. 2000

机译：结合DNA标记的脂质体生物传感设备和酶联斑点印迹分析技术的开发，结合基于核酸序列的扩增，可快速检测出产志贺毒素的大肠埃希菌。

6. Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients [O] . Lucio Vera-Cabrera, Adrian Rendon, Manuel Diaz-Rodriguez, 1999

机译：点印迹法检测抗二乙酰海藻糖结核病患者的抗体

7. Preliminary study for the detection of pig specific antibodies against peptides obtained from PRRSV envelope proteins using Dot blot assay [O] . Sanchez R Zuniga, M Czub, R Waeckerlin, 2017

机译：使用点印迹测定检测从PRRSV包膜蛋白获得的猪特异性抗体的初步研究

8. Detection of IgM Antibodies to Neisseria meningitidis Group A Polysaccharide inMeningitis Patients by Direct and Antibody Capture Enzyme-Linked Immunosorbent Assays [R] . Sippel, J. E., Girgis, N. L. 1991

机译：通过直接和抗体捕获酶联免疫吸附试验检测脑膜炎患者脑膜炎奈瑟球菌a群多糖的Igm抗体

1. 免疫印迹法检测164例结缔组织病患者抗ENA抗体结果分析 [J] . 邢忠海 ,张秀香 . 健康必读 . 2011,第010期

2. 免疫印迹法检测164例结缔组织病患者抗ENA抗体结果分析 [J] . 邢忠海 ,张秀香 . 健康必读（下旬刊） . 2011,第010期

3. 一步免疫印迹法同时检测抗RA33抗体和抗ENA抗体及其对RA患人的诊断价值 [J] . 刘学明 ,于秀明 ,于雅婷 . 中国实验诊断学 . 2005,第005期

4. ELISA法、免疫印迹法与对流免疫电泳法检测抗ENA抗体的比较 [J] . 黄文辉 ,陶怡 . 中华风湿病学杂志 . 1999,第002期

5. 免疫印迹法检测结核特异性抗体对结核病的诊断价值 [J] . 陆学东 ,孙惠平 . 中国防痨杂志 . 1999,第003期

6. 免疫色谱法抗-MPB64单克隆抗体检测结核分枝杆菌菌群方法学评价 [C] . 张嵘 ,陈功祥 ,朱百荣 . 全国检验医学感染控制和病原监测学术研讨会 . 2004

7. 酶联免疫吸附法和免疫印迹法检测抗核小体抗体的方法学比较 [A] . 朱伟涛 . 2015

1. 一种过敏原特性抗体IgG免疫印迹法检测试剂盒 [P] . 中国专利： CN206235624U . 2017.06.09

2. 基于免疫印迹法的自身抗体检测试剂盒及其测定方法 [P] . 中国专利： CN103969441A . 2014-08-06

3. Device for evaluating dot blots, useful e.g. for diagnostic detection of antibodies, comprises plate with parallel slots for blot strips and covered with optical film [P] . 外国专利： DE10061352A1 . 2002-06-13

机译：用于评估斑点印迹的装置，例如用于抗体的诊断检测，包括带有平行狭缝的板，用于印迹条并覆盖有光学膜

4. Method for the detection and measurement of Hapten-conjugated biological binding entities by western and dot-blot using anti-hapten antibodies [P] . 外国专利： US7582443B2 . 2009-09-01

机译：使用抗半抗原的抗体通过蛋白质印迹法和斑点印迹法检测和测量与半抗原结合的生物结合实体的方法

5. Method for the detection and measurement of hapten-conjugated biological binding entities by western and dot-blot using anti-hapten antibodies [P] . 外国专利： US2002132256A1 . 2002-09-19

机译：使用抗半抗原抗体通过Western和点印迹法检测和测量与半抗原结合的生物结合实体的方法

相关主题

Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients

摘要

著录项

相似文献

相关主题

期刊订阅