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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of Four Methods for Detection of Immunoglobulin M Antibodies to Dengue Virus
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Evaluation of Four Methods for Detection of Immunoglobulin M Antibodies to Dengue Virus

机译：四种检测登革热病毒免疫球蛋白M抗体的方法的评价

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Dengue has become hyperendemic in many islands of the Caribbean region. The performance in a diagnostic laboratory of four commercial assays for detection of immunoglobulin M (IgM) antibodies was evaluated. Sera from 62 patients with dengue virus infection were studied. These included 18 patients from whom dengue virus type 2 was isolated in a 1997 outbreak (specimens collected a mean of 14 days after onset of symptoms), 8 patients with dengue hemorrhagic fever (mean time after onset, 11 days), and 36 patients in whom dengue was previously confirmed by serology (mean time after onset, 10 days). Thirty serum specimens from blood donors in a country where dengue is not endemic were used as negative controls. The methods evaluated were two IgM-capture enzyme-linked immunosorbent assays (ELISA) (MRL Diagnostics, Cypress, Calif., and PanBio, Queensland, Australia), a dot ELISA dipstick assay (Integrated Diagnostics, Baltimore, Md.), and a rapid immunochromatographic assay for dengue IgG and IgM (PanBio IC). IgG antibodies were also detected by an ELISA method (MRL Diagnostics). The sensitivities of the four assays were as follows: MRL Diagnostics IgM ELISA, 98.4%; PanBio IgM ELISA, 85.5%; Integrated Diagnostics IgM dot ELISA, 96.8%; and PanBio IC, 83.9%. The specificities of all tests were 100%. Evidence of secondary dengue was found in all patients with dengue hemorrhagic fever and in 83% of the remaining patients. The MRL Diagnostics IgM ELISA appears to be more sensitive than the PanBio IgM ELISA, and this may be significant when IgM titers are low, particularly in patients with secondary dengue infections. The dot ELISA dipstick assay is equally sensitive and may be more appropriate for use in laboratories with lower workloads.
机译：登革热已在加勒比地区的许多岛屿上流行。评估了诊断实验室中用于检测免疫球蛋白M（IgM）抗体的四种商业化检测方法的性能。研究了62例登革热病毒感染患者的血清。其中包括18例在1997年暴发中分离出2型登革热病毒的患者（标本平均在症状发作后14天收集），8例登革热出血热患者（发病后平均时间11天）和36例登革热出血热患者。先前已通过血清学确诊的登革热患者（发病后的平均时间为10天）。来自登革热非流行国家的30名来自献血者的血清标本被用作阴性对照。评估的方法是两次IgM捕获酶联免疫吸附测定（ELISA）（MRL Diagnostics，Cypress，California和PanBio，Queensland，Australia），一个点ELISA量油计测定（Integrated Diagnostics，Baltimore，MD）和一个登革热IgG和IgM的快速免疫色谱分析（PanBio IC）。 IgG抗体也通过ELISA方法（MRL Diagnostics）检测。四种测定的灵敏度如下：MRL Diagnostics IgM ELISA，98.4％; PanBio IgM ELISA，85.5％;集成诊断IgM点ELISA，96.8％; PanBio IC为83.9％。所有测试的特异性均为100％。在所有登革出血热患者中以及其余83％的患者中发现了继发登革热的证据。 MRL Diagnostics IgM ELISA似乎比PanBio IgM ELISA更敏感，当IgM滴度较低时，尤其是在继发登革热感染的患者中，这可能很重要。点ELISA试纸检测法同样具有敏感性，可能更适合用于工作量较小的实验室。

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