The antibody response patterns of cattle after subcutaneous and intranasal immunizations with adhesin Tf190 of Tritrichomonas foetus were investigated. Reactions of antibody from cattle parenterally immunized with Tf190 revealed antigen specificity and Tf190 sensitization in the majority of the animals, as determined by Western blotting. The results also demonstrated strong preimmune immunoglobulin G2 (IgG2) binding to T. foetus antigens not seen in IgG1 profiles. Subcutaneous injections of Tf190 resulted in significant (P < 0.05) increases in serum IgG1 and IgG2 titers over time, as determined by parasite specific enzyme-linked immunosorbent assay. Immune sera also significantly inhibited parasite adhesion to mammalian cell lines compared to the level of inhibition obtained with preimmune sera (P < 0.05). Intranasal immunization with Tf190 failed to produce measurable parasite-specific antibody in serum; however, this immunization route did result in significant (P < 0.05) increases in parasite-specific IgA titers in cervical mucus secretions from immunized animals that were more resistant to intravaginal challenge with T. foetus than controls. These results suggest that systemic immunization with Tf190 results in serum antibody production and antiparasitic adhesin antibodies. Additionally, the results of challenge experiments with intranasally immunized animals suggests that Tf190 primes protective immune responses that lead to lower rates of infection among these animals.
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机译：研究了 Tritrichomonas foetus 的粘附素Tf190皮下和鼻内免疫后牛的抗体反应模式。如通过蛋白质印迹法所确定的，在大多数动物中肠胃外用Tf190免疫的牛的抗体反应显示出抗原特异性和Tf190敏化。结果还证明了强免疫前免疫球蛋白G2（IgG2）与 T的结合。 IgG1谱中未见到的胎儿脂肪。通过寄生虫特异性酶联免疫吸附试验确定，皮下注射Tf190会导致血清IgG1和IgG2滴度随时间显着增加（ P <0.05）。与免疫前血清获得的抑制水平相比，免疫血清还显着抑制了寄生虫对哺乳动物细胞的粘附（ P <0.05）。用Tf190进行鼻内免疫无法在血清中产生可测量的寄生虫特异性抗体。然而，这种免疫途径确实导致被免疫动物的宫颈粘液分泌物中的寄生虫特异性IgA滴度显着（ P <0.05）升高，而这些动物对 T的阴道内攻击更具抵抗力。胎儿比对照组。这些结果表明，用Tf190进行全身免疫可产生血清抗体和抗寄生虫粘附素抗体。此外，鼻内免疫动物的攻击实验结果表明，Tf190启动了保护性免疫反应，导致这些动物的感染率降低。
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