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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antibody Responses in Reindeer (Rangifer tarandus) Infected with Mycobacterium bovis
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Antibody Responses in Reindeer (Rangifer tarandus) Infected with Mycobacterium bovis

机译：牛分枝杆菌感染的驯鹿（Rangifer tarandus）的抗体反应

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Despite having a very low incidence of disease, reindeer (Rangifer tarandus) are subject to tuberculosis (TB) testing requirements for interstate shipment and herd accreditation in the United States. Improved TB tests are desperately needed, as many reindeer are falsely classified as reactors by current testing procedures. Sera collected sequentially from 11 (experimentally) Mycobacterium bovis-infected reindeer and 4 noninfected reindeer were evaluated by enzyme-linked immunosorbent assay (ELISA), immunoblotting, and multiantigen print immunoassay (MAPIA) for antibody specific to M. bovis antigens. Specific antibody was detected as early as 4 weeks after challenge with M. bovis. By MAPIA, sera were tested with 12 native and recombinant antigens, which were used to coat nitrocellulose. All M. bovis-infected reindeer developed responses to MPB83 and a fusion protein, Acr1/MPB83, and 9/11 had responses to MPB70. Other antigens less commonly recognized included MPB59, ESAT-6, and CFP10. Administration of purified protein derivatives for skin testing boosted serum antibody responses, as detected by each of the assays. Of the noninfected reindeer, 2/4 had responses that were detectable immediately following skin testing, which correlated with pathological findings (i.e., presence of granulomatous lesions yet the absence of acid-fast bacteria). The levels of specific antibody produced by infected reindeer appeared to be associated with disease progression but not with cell-mediated immunity. These findings indicate that M. bovis infection of reindeer elicits an antibody response to multiple antigens that can be boosted by skin testing. Serological tests using carefully selected specific antigens have potential for early detection of infections in reindeer.
机译：尽管疾病发病率很低，但在美国，驯鹿（ Rangifer tarandus ）仍需接受结核病（TB）测试，以确保州际间的运输和牧群认证。迫切需要改进结核病检测，因为许多驯鹿被当前的检测程序错误地归类为反应堆。通过酶联免疫吸附测定（ELISA），免疫印迹和多抗原印迹免疫测定（MAPIA）对从11个（实验）牛分枝杆菌感染的驯鹿和4个未感染驯鹿中依次收集的血清进行评估，以评估对< em> M。牛的抗原。最早在用 M攻击后4周就检测到特异性抗体。牛羊。通过MAPIA，用12种天然抗原和重组抗原测试了血清，这些抗原被用于包被硝酸纤维素。所有 M。牛感染的驯鹿对MPB83有反应，融合蛋白Acr1 / MPB83和9/11对MPB70有反应。其他不太常见的抗原包括MPB59，ESAT-6和CFP10。如每种测定所检测的，施用纯化的蛋白衍生物进行皮肤测试可增强血清抗体反应。在未感染的驯鹿中，有2/4的反应在皮肤测试后立即可检测到，这与病理结果相关（即存在肉芽肿性病变但不存在耐酸细菌）。感染的驯鹿产生的特异性抗体水平似乎与疾病进展有关，但与细胞介导的免疫无关。这些发现表明 M。牛的牛感染可引起对多种抗原的抗体反应，皮肤测试可增强这种反应。使用精心挑选的特定抗原进行血清学检测，有可能早期发现驯鹿中的感染。

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