Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09)

B. D. Kirkpatrick; Matthew D. Bentley; Anette M. Thern; Catherine J. Larsson; Cassandra Ventrone; Meera V. Sreenivasan

首页> 外文期刊>Clinical and diagnostic laboratory immunology >Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09)

【24h】

Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09)

机译：淋巴细胞上清液和抗体分泌细胞测定抗体对新型口服伤寒疫苗肠道粘膜免疫反应的测定方法比较（M01ZH09）

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Antibody-secreting cell (ASC) and antibodies in lymphocyte supernatant (ALS) assays are used to assess intestinal mucosal responses to enteric infections and vaccines. The ALS assay, performed on cell supernatants, may represent a convenient alternative to the more established ASC assay. The two methods, measuring immunoglobulin A to Salmonella enterica serovar Typhi lipopolysaccharide, were compared in volunteers vaccinated with a live-attenuated typhoid vaccine M01ZH09. The specificity of the ALS assay compared to the ASC assay was excellent (100%), as was sensitivity (82%). The ALS assay was less sensitive than the ASC assay at ≤42 spots/106 peripheral blood lymphocytes.

机译：淋巴细胞上清液（ALS）分析中的抗体分泌细胞（ASC）和抗体用于评估肠粘膜对肠道感染和疫苗的反应。在细胞上清液上进行的ALS分析可能代表了更成熟的ASC分析的便利替代方法。在接种减毒活伤寒疫苗M01ZH09的志愿者中，比较了两种方法的免疫球蛋白A对肠炎沙门氏菌血清型伤寒脂多糖的测定。与ASC分析相比，ALS分析的特异性极佳（100％），灵敏度也很高（82％）。 ≤42点/ 10 6 外周血淋巴细胞的ALS检测灵敏度不如ASC检测。

著录项

来源
《Clinical and diagnostic laboratory immunology》 |2005年第9期|共3页
作者
B. D. Kirkpatrick; Matthew D. Bentley; Anette M. Thern; Catherine J. Larsson; Cassandra Ventrone; Meera V. Sreenivasan;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类诊断学;
关键词

相似文献

外文文献
中文文献
专利

1. Robust gut associated vaccine-specific antibody-secreting cell responses are detected at the mucosal surface of Bangladeshi subjects after immunization with an oral killed bivalent V. cholerae O1/O139 whole cell cholera vaccine: Comparison with other mucosal and systemic responses. [J] . Shamsuzzaman S, Ahmed T, Mannoor K, Vaccine . 2009,第9期

机译：在口服灭活的二价霍乱弧菌O1 / O139全细胞霍乱疫苗免疫后，在孟加拉国受试者的粘膜表面检测到了与肠道相关的强健的疫苗特异性抗体分泌细胞应答：与其他粘膜和全身应答的比较。
2. Comparative evaluation of the antibody in lymphocyte supernatant (ALS) and enzyme-linked immunospot (ELISPOT) assays for measuring mucosal immune responses to Shigella antigens [J] . Andrea J. Feller, Robin McKenzie, David N. Taylor, Vaccine . 2011,第47期

机译：淋巴细胞上清液（ALS）和酶联免疫斑点（ELISPOT）测定抗体的黏膜对志贺氏菌抗原的粘膜免疫反应的比较评估
3. Comparison of the antibody in lymphocyte supernatant (ALS) and ELISPOT assays for detection of mucosal immune responses to antigens of enterotoxigenic Escherichia coli in challenged and vaccinated volunteers [J] . Carpenter CM, Hall ER, Randall R, Vaccine . 2006,第18期

机译：淋巴细胞上清液（ALS）和ELISPOT分析中抗体的比较，用于检测在挑战和接种疫苗的志愿者中对肠毒素的大肠杆菌抗原的粘膜免疫反应
4. Comparison of DLI~R controlled immunity, Enterisol~R and Porcilis~R Ileitis vaccines in grower pigs challenged with a Lawsonia intracellulars mucosal homogenate [C] . Annual Meeting of the American Association of Swine Veterinarians . 2018

机译：DLI〜R受控免疫，肠溶液与PorInis〜R inleitis患者在种植猪中致致训练的刘肠瘤均匀化
5. ANTIBODY-SECRETING CELL PRECURSOR FREQUENCIES AMONG THE SHEEP ERYTHROCYTE-BINDING CELLS OF NON-IMMUNE AND IMMUNE MICE [D] . MARTINEZ-MAZA, OTONIEL 1981

机译：非免疫和免疫小鼠的绵羊红细胞结合细胞中可分泌抗体的细胞前体频率
6. Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09) [O] . B. D. Kirkpatrick, Matthew D. Bentley, Anette M. Thern, 2005

机译：淋巴细胞上清液和抗体分泌细胞测定抗体对新型口服伤寒疫苗肠道粘膜免疫应答的测定方法比较（M01ZH09）
7. Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09) [O] . Kirkpatrick, B. D., Bentley, Matthew D., Thern, Anette M., 2005

机译：淋巴细胞上清液和抗体分泌细胞测定抗体对新型口服伤寒疫苗肠道粘膜免疫应答的测定方法比较（M01ZH09）

Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09)

摘要

著录项

相似文献

相关主题

期刊订阅