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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Naturally Acquired Antibody Responses to the C-Terminal Region of Merozoite Surface Protein 1 of Plasmodium vivax in Korea
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Naturally Acquired Antibody Responses to the C-Terminal Region of Merozoite Surface Protein 1 of Plasmodium vivax in Korea

机译：对韩国间日疟原虫裂殖子表面蛋白1 C末端区域的自然获得性抗体应答。

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We expressed a protein in Saccharomyces cerevisiae in order to evaluate the humoral immune responses to the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax. This protein (Pv20018) had a molecular mass of 18 kDa and was reactive with the sera of individuals with patent vivax malaria on immunoblotting analysis. The levels of immunoglobulin M (IgM) and IgG antibodies against Pv20018 were measured in 421 patients with vivax malaria (patient group), 528 healthy individuals from areas of nonendemicity (control group 1), and 470 healthy individuals from areas of endemicity (control group 2), using the indirect enzyme-linked immunosorbent assay (ELISA) method. To study the longevity of the antibodies, 20 subjects from the patient group were also tested for the antibody levels once a month for 1 year. When the cutoff values for seropositivity were determined as the mean + 3 × standard deviation of the antibody levels in control group 1, both IgG and IgM antibody levels were negative in 98.5% (465 of 472) of control group 2. The IgG and IgM antibodies were positive in 88.1% (371 of 421) and 94.5% (398 of 421) of the patient group, respectively. The IgM antibody became negative 2 to 4 months after the onset of symptoms, whereas the IgG antibody usually remained positive for more than 5 months. In conclusion, indirect ELISA using Pv20018 expressed in S. cerevisiae may be a useful diagnostic method for vivax malaria.
机译：我们在酿酒酵母中表达一种蛋白，以评估对间日疟原虫裂殖子表面蛋白1 C端区域的体液免疫反应。该蛋白（Pv200 18 ）的分子质量为18 kDa，在免疫印迹分析中可与间日疟原虫疟疾患者的血清反应。在421名间日间疟疾患者（患者组），528名来自非传染病地区的健康人（对照组1）和470名健康人中测量了针对Pv200 18 的免疫球蛋白M（IgM）和IgG抗体水平使用间接酶联免疫吸附测定（ELISA）方法对来自流行地区（对照组2）的个体进行检测。为了研究抗体的寿命，还对来自患者组的20名受试者进行了为期1年每月一次的抗体水平测试。当将血清阳性的临界值确定为对照组1中抗体水平的平均值+ 3×标准偏差时，对照组2中98.5％（472的465）中IgG和IgM抗体水平均为阴性。IgG和IgM抗体组分别有88.1％（421的371）和94.5％（421的398）呈阳性。症状发作后2至4个月，IgM抗体变为阴性，而IgG抗体通常保持阳性超过5个月。总之，使用 S中表达的Pv200 18 的间接ELISA。酿酒酵母可能是诊断间日疟的有效方法。

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