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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Dose-Dependent Circulating Immunoglobulin A Antibody-Secreting Cell and Serum Antibody Responses in Swedish Volunteers to an Oral Inactivated Enterotoxigenic Escherichia coli Vaccine
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Dose-Dependent Circulating Immunoglobulin A Antibody-Secreting Cell and Serum Antibody Responses in Swedish Volunteers to an Oral Inactivated Enterotoxigenic Escherichia coli Vaccine

机译：瑞典志愿者对口服灭活的产肠毒素的大肠杆菌疫苗的剂量依赖性循环免疫球蛋白A抗体分泌细胞和血清抗体反应。

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The immunogenicity of different preparations of an oral inactivated enterotoxigenic Escherichia coli (ETEC) vaccine was evaluated in Swedish volunteers previously unexposed to ETEC infection. The vaccine preparations consisted of recombinant cholera toxin B subunit (CTB) and various amounts of formalin-killed whole bacteria expressing the most prevalent colonization factor antigens (CFAs). Significant immunoglobulin A (IgA) antibody-secreting cell (ASC) responses against CTB and the various CFA components were seen in a majority of volunteers after two doses of ETEC vaccine independent of the vaccine lot given. The IgA ASC responses against CTB were significantly higher after the second than after the first immunization, whereas the CFA-specific IgA ASC responses were almost comparable after the first and second doses of ETEC vaccine. Two immunizations with one-third of a full dose of CFA-ETEC bacteria induced lower frequencies of IgA ASC responses against all the different CFAs than two full vaccine doses, i.e., 63 versus 80% for CFA/I, 56 versus 70% for CS1, 31 versus 65% for CS2, and 56 versus 75% for CS4. The proportion of vaccinees responding with rises in the titer of serum IgA antibody against the various CFA antigens was also lower after immunization with the reduced dose of CFA-ETEC bacteria. These findings suggest that measurements of circulating IgA ASCs can be used not only for qualitative but also for quantitative assessments of the immunogenicity of individual fimbrial antigens in various preparations of ETEC vaccine.
机译：在以前未接触过ETEC感染的瑞典志愿者中评估了口服灭活产肠毒素的大肠杆菌（ETEC）疫苗的不同制剂的免疫原性。疫苗制剂由重组霍乱毒素B亚基（CTB）和表达最流行的定殖因子抗原（CFA）的各种福尔马林杀死的全细菌组成。接受两剂ETEC疫苗后，在大多数志愿者中均观察到针对CTB和各种CFA成分的显着的免疫球蛋白A（IgA）抗体分泌细胞（ASC）反应，与所给予的疫苗批次无关。第二次接种后，针对CTB的IgA ASC反应明显高于第一次免疫后，而在首次和第二次接种ETEC疫苗后，CFA特异性IgA ASC反应几乎可比。全剂量CFA-ETEC细菌的三分之一进行的两次免疫诱导的针对所有不同CFA的IgA ASC应答的频率要低于两种全疫苗剂量，即CFA / I分别为63对80％，CS1为56对70％ ，对于CS2为31对比65％，对于CS4为56对比75％。在用减少剂量的CFA-ETEC细菌免疫后，随着针对各种CFA抗原的血清IgA抗体效价的升高而响应的疫苗比例也更低。这些发现表明，对循环中的IgA ASC的测量不仅可以用于定性，而且可以用于评估各种ETEC疫苗制剂中单个纤维抗原的免疫原性。

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