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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Multicenter Evaluation of a Rapid and Convenient Method for Determination of Cytomegalovirus Immunoglobulin G Avidity
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Multicenter Evaluation of a Rapid and Convenient Method for Determination of Cytomegalovirus Immunoglobulin G Avidity

机译：快速，简便的方法测定巨细胞病毒免疫球蛋白G亲和力的多中心评估

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An easy, rapid, and reproducible test to distinguish residual cytomegalovirus (CMV) immunoglobulin M (IgM) antibodies from antibodies produced in primary infection could be useful, especially for pregnant women. The CMV avidity of IgG antibodies with the VIDAS automated enzyme-linked fluorescent assay and 6 M urea was evaluated in a multicenter study to differentiate between primary CMV infections and past infections or reactivations. A total of 416 serum specimens were tested: 159 specimens were from follow-up of primary infections, and 257 were from past infections. All of the specimens from primary infections collected within 4 months (17 weeks) after the onset of the infection had an avidity index lower than 0.8. An avidity index higher than 0.8 excludes a recent primary infection of less than 4 months. However, an avidity index higher than 0.8 cannot confirm all past infections, since 48 specimens (18%) from past infections had an avidity index lower than 0.8 (between 0.5 and 0.8). The exclusion capacity could be improved (96.9%) by using a cutoff of 0.7, but this index would decrease the specificity of the technique, since the avidity index was found to be between 0.7 and 0.8 in two patients with recent primary infection. All specimens from primary infections obtained more than 4 months after the onset of infection had an avidity index more than 0.2. In this study, an avidity index less than 0.2 confirms the presence of a recent primary infection of less than 4 months. The VIDAS CMV IgG avidity test is a rapid, reproducible test with very good performance.
机译：一项简单，快速且可重复的测试可用于区分残留的巨细胞病毒（CMV）免疫球蛋白M（IgM）抗体与原发感染中产生的抗体，这对孕妇尤其有用。在一项多中心研究中，通过VIDAS自动化酶联荧光分析和6 M尿素评估了IgG抗体的CMV亲和力，以区分原发性CMV感染与既往感染或再激活。总共测试了416个血清样本：159个样本来自原发感染的随访，其中257个样本来自过去的感染。感染发生后4个月（17周）内收集的所有原发感染标本的亲和力指数均低于0.8。亲和力指数高于0.8排除了最近的少于4个月的原发感染。但是，亲和力指数高于0.8不能确认所有过去的感染，因为来自过去感染的48个样本（18％）的亲和力指数低于0.8（介于0.5和0.8之间）。通过使用0.7的临界值可以提高排斥能力（96.9％），但是该指数会降低该技术的特异性，因为发现两名原发感染的患者的亲和力指数在0.7到0.8之间。感染开始后4个月以上获得的所有原发感染标本的亲和力指数均大于0.2。在这项研究中，亲和力指数小于0.2证实了最近的原发感染少于4个月的存在。 VIDAS CMV IgG亲和力测试是一种快速，可重复的测试，性能非常好。

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