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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Apoptosis in T-Lymphocyte Subsets in Human Immunodeficiency Virus-Infected Children Measured Immediately Ex Vivo and following In Vitro Activation
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Apoptosis in T-Lymphocyte Subsets in Human Immunodeficiency Virus-Infected Children Measured Immediately Ex Vivo and following In Vitro Activation

机译：立即从体内和体外激活后测量的人类免疫缺陷病毒感染的儿童中T淋巴细胞亚群的凋亡

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摘要

Phosphatidylserine molecules are translocated to the outer plasma membrane of lymphocytes undergoing apoptosis and can be detected by the binding of fluorochrome-conjugated annexin V. Using the annexin V assay, we examined CD4 and CD8 T cells from human immunodeficiency virus (HIV)-infected children for apoptosis upon isolation or following in vitro culture. Immediate ex vivo analysis or overnight culture showed significantly higher levels of apoptosis in CD8 cells than in CD4 cells. Following culture with the activating stimulus phytohemagglutinin or anti-CD3 monoclonal antibody, we observed an increase in the percentage of apoptotic CD4 cells, whereas there was no change in the rate of CD8 cell death. These results demonstrate that in HIV-infected children, CD8 apoptosis may occur at a greater rate than CD4 apoptosis in vivo; greater CD4 depletion may be observed due to more efficient mechanisms for peripheral lymphocyte replacement in the CD8 compartment. Furthermore, our data suggest that CD8 lymphocytes may be maximally activated in vivo, a condition which may lead to the exhaustion of CD8-mediated immunity. These findings clarify the differences between the CD4 and CD8 apoptotic responses to HIV.
机译：磷脂酰丝氨酸分子易位至经历凋亡的淋巴细胞的外质膜，并且可以通过结合荧光染料的膜联蛋白V进行检测。使用膜联蛋白V分析，我们检查了感染人免疫缺陷病毒（HIV）的儿童的CD4和CD8 T细胞用于分离或体外培养后的凋亡。立即离体分析或过夜培养显示，CD8细胞中的凋亡水平明显高于CD4细胞中。用激活的刺激性植物血凝素或抗CD3单克隆抗体培养后，我们观察到凋亡CD4细胞百分比增加，而CD8细胞死亡率没有变化。这些结果表明，在HIV感染的儿童中，CD8细胞凋亡的发生率可能高于体内CD4细胞凋亡的发生率。由于更有效的CD8区室周围淋巴细胞置换机制，可以观察到更大的CD4消耗。此外，我们的数据表明，CD8淋巴细胞可能在体内被最大程度地激活，这种情况可能导致CD8介导的免疫力衰竭。这些发现阐明了CD4和CD8对HIV的凋亡反应之间的差异。

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