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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Two Methods for Rapid Serological Diagnosis of Acute Leptospirosis
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Two Methods for Rapid Serological Diagnosis of Acute Leptospirosis

机译：急性钩端螺旋体病快速血清学诊断的两种方法

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Leptospirosis is a common and underdiagnosed zoonosis. Two rapid assays for serological diagnosis of acute leptospirosis in diagnostic laboratories, the immunoglobulin M (IgM)-dipstick assay and the indirect hemagglutination assay (IHA), were evaluated and compared with standard assays. Sera were examined from 104 patients admitted to a hospital for investigation in a leptospirosis diagnostic protocol. Specimens for serology were taken on days 1 and 4 of the patients' hospital stay. Antibodies were detected using an IgM-enzyme-linked immunosorbent assay (ELISA), microscopic agglutination test (MAT), an IgM-dipstick assay, and an IHA. Fifty-one patients were found to have leptospirosis. The sensitivity of the IgM-dipstick assay was 98%, its specificity was 90.6%, its positive predictive value was 90.9%, and its negative predictive value was 98%. The sensitivity of the IHA was 92.2%, its specificity was 94.4%, its positive predictive value was 95.9%, and its negative predictive value was 92.7%. The standard IgM-ELISA and MAT, were positive in the first samples tested from 67 and 55% of the cases, respectively, and the rapid IgM-dipstick assay and IHA were positive in 71 and 49%, respectively, in the first sample tested. Both rapid assays are highly sensitive and specific. Neither requires specialized equipment, and both are suitable for use in diagnostic laboratories.
机译：钩端螺旋体病是一种常见且诊断不足的人畜共患病。在诊断实验室中，对两种用于急性钩端螺旋体病血清学诊断的快速测定进行了评估，分别是免疫球蛋白M（IgM）-试纸法和间接血凝测定（IHA），并与标准测定法进行了比较。在钩端螺旋体病诊断方案中，对104名入院接受调查的患者的血清进行了检查。在患者住院的第1天和第4天采集血清学标本。使用IgM酶联免疫吸附测定（ELISA），显微凝集试验（MAT），IgM试纸条测定和IHA检测抗体。发现五十一例患有钩端螺旋体病。 IgM-试纸检测的灵敏度为98％，特异性为90.6％，阳性预测值为90.9％，阴性预测值为98％。 IHA的敏感性为92.2％，特异性为94.4％，阳性预测值为95.9％，阴性预测值为92.7％。在分别测试的67％和55％的病例中，标准IgM-ELISA和MAT均为阳性，在进行测试的第一份样品中，快速IgM-试纸法和IHA分别为71％和49％为阳性。 。两种快速测定法都是高度敏感和特异性的。两者都不需要专用设备，并且都适合在诊断实验室中使用。

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