Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immunized against the Protozoan Parasite Ichthyophthirius multifiliis

Joanne L. Maki; Harry W. Dickerson

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Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immunized against the Protozoan Parasite Ichthyophthirius multifiliis

机译：针对原生动物寄生虫多丝鱼的免疫的Channel鱼的全身和皮肤粘液抗体反应。

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Fish acquire protective immunity against the ciliated protozoan parasite Ichthyophthirius multifiliis following sublethal infection or inoculation with I. multifiliis immobilization antigens (i-antigens). In both cases, parasite-immobilizing antibodies have been identified in sera and mucosal secretions. To investigate the kinetics of this immune response, antibody levels were determined by enzyme-linked immunosorbent assay (ELISA) in the sera and cutaneous mucus of channel catfish (Ictalurus punctatus) that were either infected with parasites or given a single injection of purified i-antigen (5.0 μg/fish) in Freund's incomplete adjuvant. At 5 weeks, infected and inoculated fish had a mean serum (1:80 dilution) antibody absorbance (A₄₀₅) value of 0.54 ± 0.17 and 0.35 ± 0.03, respectively, which were significantly higher (α = 0.05) than the pretreatment serum (1:80 dilution) antibody absorbance value of 0.24 ± 0.05. At 14 weeks, mean serum (1:80 dilution) ELISA absorbance values in the teo groups of fish increased to 0.79 ± 0.30 and 0.71 ± 0.24, respectively. In both groups of fish, antibody levels in cutaneous mucus (undiluted) were much lower than those in sera. Infected fish had detectable mucus (undiluted) antibody levels from 3 to 9 weeks, with the highest mean value (0.30 ± 0.07) occurring at 7 weeks. Although individual inoculated fish produced serum antibody absorbance values comparable to those seen in infected fish, the mean mucus antibody values in this group did not rise above pretreatment levels. I. multifiliis infection induced a transient mucosal antibody response that coincided with the resolution of infection. Whether elicited by infection or intraperitoneal injection of i-antigen, the serum and mucus antibody responses of channel catfish immunized against I. multifiliis did not occur synchronously.

机译：亚致死性感染或接种 I后，鱼对纤毛的原生动物寄生虫具有保护性免疫。多丝固定抗原（i-抗原）。在这两种情况下，已在血清和粘膜分泌物中鉴定出了固定寄生虫的抗体。为了研究这种免疫反应的动力学，通过酶联免疫吸附测定（ELISA）测定了感染或寄生虫的given鱼（ Ictalurus punctatus ）的血清和皮肤黏液中的抗体水平在弗氏不完全佐剂中单次注射纯化的i抗原（5.0μg/鱼）。在第5周时，感染和接种的鱼的平均抗体吸收率（ A _{405 ）值（1:80稀释）分别为0.54±0.17和0.35±0.03，显着高于预处理血清（1:80稀释）的抗体吸光度值0.24±0.05（α= 0.05）。在第14周时，鱼的teo组的平均ELISA（1:80稀释度）ELISA吸光度值分别增加到0.79±0.30和0.71±0.24。在两组鱼类中，皮肤粘液（未稀释）中的抗体水平均远低于血清中的抗体水平。受感染的鱼在3至9周内可检测到的粘液（未稀释）抗体水平，在7周时出现最高平均值（0.30±0.07）。尽管单独接种的鱼产生的血清抗体吸收值可与感染鱼中观察到的值相提并论，但该组中的平均粘液抗体值未超过预处理水平。我multifiliis 感染诱导了短暂的粘膜抗体反应，与感染的消退相吻合。无论是由于感染还是腹膜内注射i抗原引起的，针对 I免疫的channel鱼的血清和粘液抗体反应。 multifiliis 没有同步发生。} 展开▼

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《Clinical and diagnostic laboratory immunology》 |2003年第5期|共6页

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Joanne L. Maki; Harry W. Dickerson;
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专利

1. Passive immunization of channel catfish (Ictalurus punctatus) against the ciliated protozoan parasite Ichthyophthirius multifiliis by use of murine monoclonal antibodies. [J] . T L Lin, T G Clark, H Dickerson Infection and immunity . 1996,第10期

机译：通过使用鼠类单克隆抗体针对纤毛的原生动物寄生虫Ichthyophthirius multifiliis对cat鱼（Ictalurus punctatus）进行被动免疫。

2. Comparison of serum antibody responses and host protection against parasite Ichthyophthirius multifiliis between channel catfish and channel x blue hybrid catfish [J] . De-Hai Xu, Phillip H. Klesius Fish & Shellfish Immunology . 2013,第5期

机译：channel鱼和蓝x杂交hybrid鱼的血清抗体应答和宿主对寄生虫多丝鱼的保护作用比较

3. Effect of immunization of channel catfish with inactivated trophonts on serum and cutaneous antibody titers and survival against Ichthyophthirius multifiliis [J] . Xu DH, Klesius PH, Shoemaker CA Fish & Shellfish Immunology . 2009,第4期

机译：灭活的刺苗对河豚cat的免疫对血清和皮肤抗体滴度及抵抗多丝鱼的存活的影响

4. IMMUNIZATION OF GUPPIES (POECILIA RETICULATA) AGAINST THE PROTOZOAN PARASITE TETRAHYMENA SP [C] . Jiwan K. Chettri, M. Pimenta Leibowitz, D. Zilberg International Conference of the European Aquaculture Society . 2008

机译：对抗原生动物寄生虫Tetrahymena SP的Guppies（Poecilia TeariCulata）的免疫

5. Laboratory infection of zebrafish (Danio rerio) and channel catfish (Ictalurus punctatus) with the protozoan parasite Ichthyophthirius multifiliis: A model for parasite persistence. [D] . Cherry, Bryan. 2003

机译：斑马鱼（Danio rerio）和斑channel鱼（Ictalurus punctatus）的实验室感染有原生动物寄生虫Ichthyophthirius multifiliis：寄生虫持久性的模型。

6. Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immunized against the Protozoan Parasite Ichthyophthirius multifiliis [O] . Joanne L. Maki, Harry W. Dickerson 2003

机译：针对原生动物寄生虫多丝鱼的免疫的Channel鱼的全身和皮肤粘液抗体反应。

7. Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immunized against the Protozoan Parasite Ichthyophthirius multifiliis [O] . Maki, Joanne L., Dickerson, Harry W. 2003

机译：针对原生动物寄生虫多丝鱼的免疫的Channel鱼的全身和皮肤粘液抗体反应。

8. Management, Prevention and Treatment of Ichthyophthirius Multifilis in the211 Intensive Culture of Walleye and Channel Catfish. Federal Aid to Fish Restoration 211 Completion Report. Fisheries Research [R] . 1998

机译：作者：王莹，王莹，王莹，王莹，中国农业科学sCIENTIa联邦援助鱼类恢复211完成报告。渔业研究

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2. 荧光抗体技术检查尿沉渣粘液丝免疫球蛋白及其临床意义 [J] . 伍志刚 ,吴轰 ,谢景超 . 中华肾脏病杂志 . 1990,第003期

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6. ROR和ROR参与硬骨鱼免疫反应的初步研究 [C] . 杜林勇 ,杨璐 ,杨潇 . 中国海洋湖沼学会、中国动物学会鱼类学分会2012年学术研讨会 . 2012

7. RT97识别的神经丝蛋白在斑马鱼视网膜和脑发生过程中表达的免疫组化研究 [A] . 王芳春 . 2007

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2. 通过直接向鱼施加电去除寄生虫而不伤害鱼的用于去除粘附在鱼身上的寄生虫的系统 [P] . 中国专利： CN110248542A . 2019-09-17

3. Composition of vaccine pharmaceutical product processes to immunize a carrier against influenza infection to optimize an immune response of protective iga mucosa and a systemic immune response and igg and to enhance the immune response of influenza virus antigens and use of a chitosan [P] . 外国专利： BR9509190A . 1997-10-21

机译：疫苗载体产品工艺的组成，该工艺可对流感病毒感染的载体进行免疫，以优化保护性iga粘膜的免疫反应以及全身性免疫反应和igg，并增强流感病毒抗原的免疫反应和壳聚糖的用途

4. Nucleic acid molecule, recombinant vector, polypeptide, eukaryotic amino acid racemase, antibodies, host cell, methods to produce polypeptide, to detect a parasite and a eukaryotic protein, to screen active molecules, to detect and quantify the presence or absence of a sequence, to inhibit a eukaryotic protein, to produce a eukaryotic and d-aminoacid recombinant amino acid racemase and to prevent or inhibit infection, plasmid, immune complex, kit to detect a parasite, fragment of a polynucleotide, eukaryotic protein, processes to prepare a purified eukaryotic protein, to detect an infection and for infection and to screen a molecule, immunizing composition, vaccine composition, any molecular modification of the gene or a fragment of the gene, use of any molecular or biochemical modification of the enzymatic activity of the racemase, and , any molecule or composition to [P] . 外国专利： BR0016129A . 2002-08-20

机译：核酸分子，重组载体，多肽，真核氨基酸消旋酶，抗体，宿主细胞，生产多肽，检测寄生虫和真核蛋白质，筛选活性分子，检测和定量序列存在或不存在的方法，抑制真核蛋白，产生真核和d-氨基酸重组氨基酸消旋酶并预防或抑制感染，质粒，免疫复合物，检测寄生虫，多核苷酸片段，真核蛋白的试剂盒，制备纯化的真核生物的方法蛋白质，以检测感染和感染，并筛选分子，免疫组合物，疫苗组合物，基因或基因片段的任何分子修饰，消旋酶酶活性的任何分子或生化修饰，以及，任何分子或组成

5. Channel catfish virus disease vaccine and method of preparation thereof and method of immunization therewith [P] . 外国专利： US4219543A . 1980-08-26

机译：鱼病毒病疫苗及其制备方法和免疫方法

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Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immunized against the Protozoan Parasite Ichthyophthirius multifiliis

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