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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Antigen-Presenting Cell Modulation Induces a Memory Response to p24 in Peripheral Blood Leukocytes from Human Immunodeficiency Virus-Infected Individuals
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Antigen-Presenting Cell Modulation Induces a Memory Response to p24 in Peripheral Blood Leukocytes from Human Immunodeficiency Virus-Infected Individuals

机译：抗原呈递细胞调节诱导来自人类免疫缺陷病毒感染者的外周血白细胞对p24的记忆反应

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The accurate determination of human immunodeficiency virus type 1 (HIV-1)-specific proliferative responses is critically important when evaluating immune recovery after highly active antiretroviral therapy. Using a new assay to enhance proliferative responses to recall and HIV antigen, we addressed the questions of whether viral load affects cellular immunity and whether long-term viral load suppression results in loss of antigen-specific responder cells. This assay is based on the fact that lipopolysaccharide (LPS) can augment proliferative responses to antigen after monocyte adherence to a tissue culture plate. Twenty-six HIV-1-infected individuals donated peripheral blood leukocytes (PBL). Proliferation assays against p24, using LPS and cell adherence, were performed on all samples. Medical record abstraction provided information on CD4 cell nadir and time of viral load suppression. PBL from HIV-1-infected individuals with a viral load of <200 copies/ml had a significant proliferative response and a stimulation index of >5 to p24 (12 of 15) compared to those with a viral burden (2 of 11), using the LPS-adherence assay. Proliferative responses to p24 could be found in PBL from virally suppressed donors independent of the CD4 cell nadirs and in the majority of the donors who were virally suppressed for >10 months (7 of 10). The data presented here demonstrate that LPS and monocyte adherence provide a sensitive and specific way to boost proliferative responses to recall and HIV antigens.
机译：当评估高活性抗逆转录病毒疗法后的免疫恢复时，准确测定人类1型免疫缺陷病毒（HIV-1）特异性增殖反应至关重要。使用一种新的测定方法来增强对召回和HIV抗原的增殖反应，我们解决了病毒载量是否影响细胞免疫以及长期病毒载量抑制是否导致抗原特异性应答细胞丢失的问题。该测定基于以下事实：单核细胞粘附到组织培养板上后，脂多糖（LPS）可以增强对抗原的增殖反应。 26名受HIV-1感染的人捐赠了外周血白细胞（PBL）。在所有样品上进行了使用LPS和细胞粘附的针对p24的增殖分析。病历摘要提供了有关CD4细胞最低点和病毒载量抑制时间的信息。与病毒载量（11之2）相比，来自HIV-1感染者的PBL的病毒载量小于200拷贝/毫升，具有显着的增殖反应，对p24的刺激指数大于5，对p24（15分中的12分），使用LPS粘附测定。独立于CD4细胞最低点的被病毒抑制的供体和大多数被病毒抑制10个月以上的供体（共10个中的7个）可在PBL中发现对p24的增殖反应。此处提供的数据表明，LPS和单核细胞的粘附提供了灵敏且特异的方式来增强对召回和HIV抗原的增殖反应。

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