...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evaluation of Five Antibody Detection Tests for Diagnosis of Bovine Paratuberculosis
【24h】

Evaluation of Five Antibody Detection Tests for Diagnosis of Bovine Paratuberculosis

机译：五种抗体检测方法对牛副结核病的诊断价值

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Five diagnostic tests based on enzyme-linked immunosorbent assay (ELISA) technology for bovine paratuberculosis were evaluated by using individual serum or milk samples from 359 dairy cattle in seven paratuberculosis-free herds and 2,094 dairy cattle in seven Mycobacterium paratuberculosis-infected dairy herds. Three independent laboratories using three different culture procedures completed fecal cultures for M. paratuberculosis on these cattle and found 417 cows to be shedding M. paratuberculosis in their feces. An animal that was fecal culture positive for M. paratuberculosis by any of the three laboratories was considered a confirmed case of infection. The specificity of three ELISAs (two on serum and one on milk) was ≥99.8%. The specificity of the remaining two ELISAs, both done on serum, was 94.9 and 84.7%. Four of the five ELISAs evaluated produced similar sensitivity in detecting fecal culture-positive cattle (27.8 to 28.9%). Serum ELISA “D” had the lowest specificity (84.7%) and the highest sensitivity (44.5%), but if the cutoff value defining a positive test was changed from 125 to 250% (of the positive control) the sensitivity and specificity, 31.8 and 97.5%, respectively, were comparable to those of the other four assays. If the case definition for M. paratuberculosis infection was based on the culture results of a single laboratory instead of the combined results of three laboratories, ELISA sensitivity estimates were 45.7 to 50.0%. With the exception of ELISA D, assay agreement was high (kappa 0.66 to 0.85) for categorical assay interpretations (positive or negative), but linear regression of quantitative results showed low correlation coefficients (r2 = 0.40 to 0.68) due to the fact that ELISA results for some cows were high in one assay but low in another assay. Likelihood ratio analysis showed a direct relationship between the magnitude of ELISA result and the odds of a cow shedding M. paratuberculosis in its feces. If used judiciously and interpreted quantitatively, these ELISAs are useful tools in support of paratuberculosis control programs in dairy herds.
机译：通过使用酶联免疫吸附测定（ELISA）技术对牛副结核病的五项诊断测试进行了评估，方法是使用七个无副结核病牛群中的359头奶牛和七个副结核分枝杆菌中的2,094头奶牛的血清或牛奶样品>-感染的奶牛群。使用三个不同培养程序的三个独立实验室完成了 M的粪便培养。在这些牛身上发现副结核病，发现417头牛正在流产 M。粪便中的肺结核。粪便培养 M呈阳性的动物。三个实验室中的任何一个实验室都认为结核菌是副结核病。三种ELISA（血清中两种，牛奶中一种）的特异性≥99.8％。其余两种ELISA的特异性均在血清上进行，分别为94.9和84.7％。评估的5种ELISA中有4种在检测粪便培养阳性牛中产生了相似的灵敏度（27.8％至28.9％）。血清ELISA“ D”具有最低的特异性（84.7％）和最高的敏感性（44.5％），但是如果定义阳性试验的标准值从（阳性对照的）125％变为250％，则敏感性和特异性为31.8分别为97.5％和97.5％，与其他四种测定的结果相当。如果 M的大小写定义。副结核菌感染是基于单个实验室的培养结果而不是三个实验室的合并结果，ELISA灵敏度估计为45.7至50.0％。除ELISA D外，分类分析的分析一致性（正或负）均较高（kappa为0.66至0.85），但是定量结果的线性回归显示相关系数较低（ r 2 = 0.40至0.68），因为某些母牛的ELISA结果在一种测定中较高，而在另一种测定中较低。似然比分析显示ELISA结果的大小与母牛脱落 M的几率之间存在直接关系。粪便中的肺结核。如果明智地使用并定量地解释，这些ELISAs是支持乳牛群副结核病控制计划的有用工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号