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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Preliminary Evaluation of Whole-Blood Gamma Interferon Release for Clinical Assessment of Cellular Immunity in Patients with Active Coccidioidomycosis
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Preliminary Evaluation of Whole-Blood Gamma Interferon Release for Clinical Assessment of Cellular Immunity in Patients with Active Coccidioidomycosis

机译：全血γ干扰素释放对活动性球孢菌病患者细胞免疫性临床评估的初步评估

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Assessment of the cellular immune response in coccidioidomycosis has epidemiologic and prognostic importance. Measurement of delayed-type hypersensitivity to skin testing has been used in the past to determine cellular immunity in coccidioidomycosis. However, no skin tests are currently available in the United States. Assay of gamma interferon (IFN-γ) release in whole blood in response to incubation with antigen has been used to assess cellular immunity in tuberculosis. We used a similar assay using the coccidioidal antigen preparation T27K to measure the in vitro cellular immune responses among a cohort of 69 subjects with active coccidioidomycosis. IFN-γ release was bimodal, with concentrations above and below 5 IU/ml. Using multivariate logistic regression, underlying disease and disseminated or chronic pulmonary coccidioidomycosis was significantly associated with the release of IFN-γ at a concentration of <5 IU/ml (P = 0.02 or 0.05, respectively). In addition, the release IFN-γ concentration was <5 IU/ml in all subjects with a clinical severity score of ≥6 (P = 0.02). The release IFN-γ concentration correlated with expression of CD69 on T lymphocytes in an in vitro assay using T27K as the antigen (Spearman's rho = 0.59; P < 0.01). These results suggest that the IFN-γ release assay with T27K as the antigen may be a useful clinical test for assessing cellular immunity in patients with active coccidioidomycosis.
机译：球孢子菌病中细胞免疫反应的评估具有流行病学和预后的重要性。过去已经使用对皮肤测试的迟发型超敏反应的测量来确定球虫病中的细胞免疫。但是，美国目前没有皮肤测试。响应于与抗原的温育，全血中γ-干扰素（IFN-γ）释放的测定已用于评估结核病中的细胞免疫。我们使用类似的测定方法，使用球状抗球虫抗原制剂T27K来测量69名活动性球虫中链球菌病患者的体外细胞免疫应答。 IFN-γ释放是双峰的，浓度高于和低于5 IU / ml。使用多元逻辑回归分析，潜在疾病和弥漫性或慢性肺球虫病与浓度<5 IU / ml的IFN-γ释放显着相关（分别为 P = 0.02或0.05）。此外，所有受试者的临床严重程度评分≥6（ P = 0.02）的释放IFN-γ浓度均<5 IU / ml。在以T27K为抗原的体外试验中，释放的IFN-γ浓度与T淋巴细胞上CD69的表达相关（Spearman的rho = 0.59; P <0.01）。这些结果表明，以T27K为抗原的IFN-γ释放测定法可能是评估活动球虫病患者细胞免疫力的有用临床测试。

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