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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Seroreactivity to and Avidity for Recombinant Antigens in Toxoplasmosis
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Seroreactivity to and Avidity for Recombinant Antigens in Toxoplasmosis

机译：对弓形虫病的重组抗原的血清反应活性和亲和力

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To improve serodiagnostic methods for the diagnosis of acute toxoplasmosis during pregnancy, a new test system has been developed and evaluated based on the use of recombinant antigens. Five recombinant Toxoplasma gondii antigens (ROP1, MAG1, SAG1, GRA7, and GRA8) were cloned in Escherichia coli, purified, and applied directly onto nitrocellulose membranes in a line assay (recomLine Toxoplasma). A panel of 102 sera from 25 pregnant women with supposed recent toxoplasmosis and from two symptomatic children was compared to a panel of 71 sera from individuals with past infection. Both panels were analyzed using a recombinant line assay for immunoglobulin G (IgG), IgM, and IgA antibodies and a reference enzyme-linked immunosorbent assay. Within the IgM-positive samples, antibodies against ROP1 were predominant regardless of the infection state. In IgG analysis a characteristic antibody pattern was found for very recent infections. This pattern changed to a different one during the time course of infection: antibodies against GRA7 and GRA8 were characteristic for very early IgG, whereas antibodies against SAG1 and MAG1 appeared significantly later. These results were further confirmed by determination of the IgG antibody avidity for every single recombinant antigen. In the time course of infection, IgG antibodies against the early recognized antigens matured significantly earlier than those directed against the later antigens did. The IgA patterns did not give reliable information about the infection time points. The data revealed that the recombinant line assay provides valuable information on the actual state of infection, especially during the early infection time points.
机译：为了改进用于诊断妊娠期间急性弓形虫病的血清诊断方法，基于重组抗原的使用，已经开发并评估了一种新的测试系统。将五种重组弓形虫抗原（ROP1，MAG1，SAG1，GRA7和GRA8）克隆到大肠杆菌中，纯化后直接用于硝酸纤维素膜上（在线检测） recom 线弓形虫）。将一组来自25名可能患有弓形虫病的孕妇和两名有症状儿童的102份血清与来自过去感染者的71份血清进行比较。使用重组品系免疫球蛋白G（IgG），IgM和IgA抗体以及参考酶联免疫吸附法对两个板块进行了分析。在IgM阳性样品中，无论感染状态如何，抗ROP1抗体都是主要抗体。在IgG分析中，发现了针对最近感染的特征性抗体模式。在感染的整个过程中，这种模式改变为另一种模式：针对GRA7和GRA8的抗体是非常早的IgG的特征，而针对SAG1和MAG1的抗体则出现得较晚。通过确定每种重组抗原的IgG抗体亲和力，进一步证实了这些结果。在感染的过程中，针对早期识别的抗原的IgG抗体比针对晚期抗原的IgG抗体显着早成熟。 IgA模式未提供有关感染时间点的可靠信息。数据显示，重组品系测定可提供有关实际感染状态的宝贵信息，尤其是在早期感染时间点。

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