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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Spontaneous lymphocyte proliferation in human T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infection: T-cell subset responses and their relationships to the presence of provirus and viral antigen production.
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Spontaneous lymphocyte proliferation in human T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infection: T-cell subset responses and their relationships to the presence of provirus and viral antigen production.

机译：I型人T细胞淋巴病毒（HTLV-I）和HTLV-II感染中的自发淋巴细胞增殖：T细胞亚群应答及其与前病毒和病毒抗原产生的关系。

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Spontaneous lymphocyte proliferation (SLP) during in vitro culture of mononuclear cells (MCs) characterizes over half of asymptomatic individuals infected with human T-cell lymphotropic virus type I (HTLV-I) or HTLV-II. Both CD4 and CD8 T-cell subsets within MC cultures are activated during SLP, as judged by high-density CD25 (CD25bright) expression; it is unclear, however, whether both cell subsets can directly undergo SLP. In the present investigation, the SLP capacities of purified CD8 and CD4 cells were examined in subjects infected with HTLV-I (n = 19) or HTLV-II (n = 54) in relation to the SLP status of MCs from each subject. No increase in SLP was observed for CD8 or CD4 cells from SLP-negative (SLP-) HTLV-infected subjects, whereas robust SLP characterized CD8 cells from all SLP-positive (SLP+) individuals, regardless of HTLV type. In contrast, SLP+ CD4 cells characterized only 23% (7 of 31) of HTLV-II+ SLP+ individuals, whereas SLP+ CD4 cells characterized 100% of HTLV-I+ SLP+ individuals. In cocultures of HTLV-II+ SLP+ CD8 cells and autologous SLP- CD4 cells, sizable proportions of both CD8 cells and CD4 cells coexpressed CD25bright, suggesting that SLP- CD4 cells were activated in the presence of SLP+ CD8 cells. PCR analysis for tax sequences detected provirus in most CD4- and CD8-cell preparations from HTLV-seropositive individuals, regardless of type and the SLP status of cell subsets. To determine whether SLP was associated with activation of viral genes, levels of HTLV-I and HTLV-II core antigen (Ag) in supernatants were measured. Viral Ag production and SLP responses were significantly correlated for both CD4 and CD8 cells in both HTLV-I and HTLV-II infections. However, inhibition of CD8- or CD4-cell SLP by cyclosporin A or anti-Tac (anti-CD25) did not reduce Ag production, indicating that Ag production is not coupled to SLP. These findings show that CD4 cells from SLP+ HTLV-I+ and SLP+ HTLV-II+ individuals differ in SLP capacity, that the absence of SLP does not indicate a lack of infection, and that production of viral Ag is associated with, but not dependent on, SLP.
机译：在单核细胞（MCs）的体外培养过程中，自发性淋巴细胞增殖（SLP）表征了一半以上的无症状个体感染了I型人T细胞淋巴病毒（HTLV-I）或HTLV-II。通过高密度CD25（CD25bright）表达判断，MC培养物中的CD4和CD8 T细胞亚群均在SLP期间被激活。然而，尚不清楚两个细胞亚群是否都可以直接进行SLP。在本研究中，检查了感染HTLV-I（n = 19）或HTLV-II（n = 54）的受试者中纯化的CD8和CD4细胞的SLP容量，与每个受试者的MC的SLP状态有关。对于来自SLP阴性（SLP-）HTLV感染受试者的CD8或CD4细胞，未观察到SLP增加，而健壮的SLP表征了来自所有SLP阳性（SLP +）个体的CD8细胞，而与HTLV类型无关。相反，SLP + CD4细胞仅表征HTLV-II + SLP +个体的23％（31个中的7个），而SLP + CD4细胞表征HTLV-I + SLP +个体的100％。在HTLV-II + SLP + CD8细胞和自体SLP- CD4细胞的共培养物中，相当大比例的CD8细胞和CD4细胞共表达CD25bright，这表明SLP- CD4细胞在存在SLP + CD8细胞的情况下被激活。对税金序列的PCR分析在HTLV血清阳性个体的大多数CD4和CD8细胞制剂中检测到前病毒，而与细胞亚群的类型和SLP状态无关。为了确定SLP是否与病毒基因的激活相关，测量了上清液中HTLV-I和HTLV-II核心抗原（Ag）的水平。在HTLV-I和HTLV-II感染中，CD4和CD8细胞的病毒Ag产生和SLP反应均显着相关。但是，环孢菌素A或抗Tac（抗CD25）抑制CD8-或CD4-细胞SLP不会减少Ag的产生，这表明Ag的产生并未与SLP偶联。这些发现表明，来自SLP + HTLV-I +和SLP + HTLV-II +个体的CD4细胞在SLP能力方面有所不同，缺少SLP并不表示缺乏感染，并且病毒Ag的产生与（但不依赖于） SLP。

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