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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Isolation and preliminary evaluation of a low-molecular-mass antigen preparation for improved detection of Helicobacter pylori immunoglobulin G antibodies.
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Isolation and preliminary evaluation of a low-molecular-mass antigen preparation for improved detection of Helicobacter pylori immunoglobulin G antibodies.

机译：低分子质量抗原制剂的分离和初步评估，用于改善幽门螺杆菌免疫球蛋白G抗体的检测。

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Previously, immunoglobulin G (IgG) antibodies to five antigens with a relative molecular mass of between 15 and 30 kDa from Helicobacter pylori were found to be significantly more frequent in H. pylori-infected patients than in noninfected patients. In this study, these specific low-molecular-mass (LMW) antigens were separated by ultrafiltration of whole-cell sonicates. The LMW antigen preparation was evaluated by enzyme-linked immunosorbent assay with serum samples from 76 children with abdominal symptoms and 151 adults with dyspeptic symptoms. H. pylori was cultured or seen in 40 (53%) children and 83 (55%) adults. Increased antibody levels to H. pylori were found in serum from 35 (46%) children and 88 (58%) adults. Values for sensitivity, specificity, and predictive value of positive and negative results of the test were higher with LMW antigens than with the heat-stable antigen previously described. The low specificity and predictive value of a positive result were due to seropositive results for 21 persons with a negative culture for H. pylori and negative microscopy results for Helicobacter-like organisms in biopsies from gastric mucosa. Histologically, chronic gastritis was demonstrated in 43% of these persons, and 19% had peptic ulcer, indicating that they have or have had H. pylori infection. Specific antibodies to H. pylori were confirmed in all 21 patients by the Western immunoblot technique. Use of the LMW antigen improved the IgG antibody detection in patients with H. pylori infection, even though the results reflect the difficulties in establishing a true gold standard for diagnosis of H. pylori infection.
机译：以前，发现来自幽门螺杆菌的相对分子质量在15至30 kDa之间的5种抗原的免疫球蛋白G（IgG）抗体在感染幽门螺杆菌的患者中比未感染的患者明显更频繁。在这项研究中，这些特异的低分子质量（LMW）抗原通过全细胞超声的超滤分离。通过酶联免疫吸附试验，对76例有腹部症状的儿童和151例消化不良症状的成人的血清样品进行了LMW抗原制剂的评估。在40名（53％）儿童和83名（55％）成人中培养或见过幽门螺杆菌。在35名（46％）儿童和88名（58％）成人的血清中发现了针对幽门螺杆菌的抗体水平升高。 LMW抗原的敏感性，特异性和试验阳性和阴性结果的预测值均高于前述的热稳定抗原。阳性结果的低特异性和预测值是由于21例幽门螺杆菌培养阴性的人出现血清反应阳性，而胃黏膜活检中幽门螺杆菌样微生物的显微镜检查结果却阴性。从组织学上看，这些人中有43％证实患有慢性胃炎，其中19％患有消化性溃疡，表明他们患有或曾经感染过幽门螺杆菌。通过Western免疫印迹技术在所有21例患者中证实了针对幽门螺杆菌的特异性抗体。 LMW抗原的使用改善了幽门螺杆菌感染患者的IgG抗体检测，即使结果反映出难以建立诊断幽门螺杆菌感染的真正金标准。

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