...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Determination of CD4 and CD8 lymphocyte subsets by a new alternative fluorescence immunoassay.
【24h】

Determination of CD4 and CD8 lymphocyte subsets by a new alternative fluorescence immunoassay.

机译：通过新的替代荧光免疫测定法测定CD4和CD8淋巴细胞亚群。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of this study was to evaluate a new alternative fluorescence immunoassay method (Zymmune CD4/CD8 Cell Monitoring Kit; Zynaxis, Inc., Malvern, Pa.) for determining the absolute CD4+ and CD8+ T-lymphocyte concentrations in whole blood. The investigation was performed as a two-site comparison of the reference whole blood flow cytometric method with the Zymmune method. In this investigation, a total of 166 patient samples were evaluated of which approximately 20% were from human immunodeficiency virus-positive individuals. The mean value for samples performed by the Zymmune CD4 assay was 1,094 (range, 74 to 2,586) cells per microliters, while the reference method yielded a mean of 890 (range, 35 to 2,033) cells per microliter. The correlation coefficient for regression analysis was 0.940. The mean value for samples performed by the Zymmune CD8 assay was 700 (range, 212 to 1,813) cells per microliter, while the reference method yielded a mean of 546 (range, 82 to 2,158) cells per microliter. The correlation coefficient for regression analysis was 0.921. No site-specific differences or trends in CD4 or CD8 values were seen when the data were analyzed by site of collection. The average precision of the CD4 assay varied from 6 to 14%, corresponding to the high and low concentration ranges. For CD8, the average precision varied from 8.3 to 16% over the respective high to low concentration ranges. We conclude that the Zymmune CD4/CD8 Cell Monitoring Kit method provides absolute CD4+ and CD8+ T-lymphocyte concentrations which are equivalent to those given by the reference flow cytometric method.
机译：这项研究的目的是评估一种新的替代荧光免疫测定方法（Zymmune CD4 / CD8细胞监测试剂盒; Zynaxis，Inc.，Malvern，PA），以确定全血中CD4 +和CD8 + T淋巴细胞的绝对浓度。作为参考全血流式细胞术与Zymmune方法的两点比较进行了研究。在这项调查中，总共评估了166个患者样本，其中约20％来自人类免疫缺陷病毒阳性个体。通过Zymmune CD4分析执行的样品的平均值为每微升1,094（范围为74至2,586）个细胞，而参考方法的平均值为每微升890（范围为35至2,033）个细胞。回归分析的相关系数为0.940。通过Zymmune CD8分析执行的样品的平均值为每微升700个细胞（范围为212至1,813个），而参考方法的平均值为每微升546个细胞（范围为82至2,158个）。回归分析的相关系数为0.921。按收集地点分析数据时，未观察到CD4或CD8值的特定地点差异或趋势。 CD4分析的平均精度在6％至14％之间变化，对应于高和低浓度范围。对于CD8，在各自的高低浓度范围内，平均精度在8.3％至16％之间变化。我们得出的结论是，Zymmune CD4 / CD8细胞监测试剂盒方法提供的CD4 +和CD8 + T淋巴细胞绝对浓度与参考流式细胞术方法所提供的绝对浓度相等。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号