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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Oral fluid as a specimen for detection and confirmation of antibodies to human immunodeficiency virus type 1.
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Oral fluid as a specimen for detection and confirmation of antibodies to human immunodeficiency virus type 1.

机译：口服液作为检测和确认1型人类免疫缺陷病毒抗体的标本

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Paired serum and oral fluid specimens (n = 287) were collected with the Omni-Sal device and were assayed for the presence of antibodies to human immunodeficiency virus type 1 (HIV-1). Enzyme immunoassays (EIAs)--Abbott 3A11, an Organon Teknika Corporation research-use-only test, and the Murex GACELISA--were used per the manufacturers' inserts or were modified slightly to accommodate the oral fluid specimens. Compared with serum Western blot (immunoblot) results, each EIA had a sensitivity of 100% and the specificities were 89.6% for the Abbott 3A11 EIA, 96.5% for the GACELISA, and 97.8% for the Organon Teknika Corporation EIA. Specificities based on specimens that were repeatedly reactive were 99.3% for all EIAs. A miniaturized Western blot technique used for confirmatory testing of both the serum and oral fluid specimens found 149 of the 287 samples to be HIV-1 antibody positive in both sample types. The Western blot banding patterns observed for the serum and oral fluid specimens were essentially identical. Immunoglobulin G concentrations were determined for all oral fluid specimens and ranged from < 0.5 to > 40.0 micrograms/ml. Immunoglobulin G concentrations did not correlate with the ability of any of the EIAs to detect HIV-1-specific antibody or with the ability of the modified Western blot to detect HIV-1 protein-specific antibodies.
机译：用Omni-Sal设备收集成对的血清和口腔液标本（n = 287），并分析是否存在针对人类1型免疫缺陷病毒（HIV-1）的抗体。酶免疫测定（EIA）-Organon Teknika Corporation的仅供研究使用的测试Abbott 3A11和Murex GACELISA-已按照制造商的说明书使用，或进行了少许改动以适应口腔液标本。与血清蛋白质印迹（免疫印迹）结果相比，每种EIA的敏感性均为100％，Abbott 3A11 EIA的特异性为89.6％，GACELISA的特异性为96.5％，Organon Teknika Corporation EIA的特异性为97.8％。对于所有EIA，基于可重复反应的标本的特异性为99.3％。用于对血清和口腔液体样品进行确认性测试的微型Western印迹技术发现，两种样品类型中287个样品中有149个HIV-1抗体呈阳性。血清和口腔液体标本中观察到的蛋白质印迹条带模式基本相同。测定所有口腔液体样品的免疫球蛋白G浓度，范围从<0.5到> 40.0微克/毫升。免疫球蛋白G的浓度与任何EIA检测HIV-1特异性抗体的能力或经修饰的Western blot检测HIV-1蛋白特异性抗体的能力均不相关。

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