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首页> 外文期刊>Clinical and diagnostic laboratory immunology >A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis
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A New Cell Enzyme-Linked Immunosorbent Assay Demonstrates Gamma Interferon Suppression by Beta Interferon in Multiple Sclerosis

机译：一种新的细胞酶联免疫吸附试验表明多发性硬化症中β干扰素对γ干扰素的抑制作用。

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摘要

Multiple sclerosis (MS) is a demyelinating disorder of the central nervous system of unknown etiology. Immune mechanisms involving the proinflammatory cytokine gamma interferon (IFN-γ) are believed to play an important role in the pathogenesis of MS. IFN-β-1b has been introduced as a treatment for MS and was found to reduce the number and severity of clinical exacerbations. To examine the influence of IFN-β-1b on myelin basic protein (MBP)-specific and phytohemagglutinin-induced IFN-γ production, we developed a cell-released capturing enzyme-linked immunosorbent assay (CRC-ELISA), which rapidly measures spontaneous and antigen- or mitogen-induced cellular IFN-γ production. CRC-ELISA documented a significant MBP-specific T-cell response in the blood of untreated MS patients, as assessed by IFN-γ production. This response was suppressed in MS patients treated with IFN-β-1b. The present work confirms in vivo the in vitro suppressive effects of IFN-β-1b on IFN-γ production in MS. Moreover, it provides a powerful new technique for detection of cytokines.
机译：多发性硬化症（MS）是病因不明的中枢神经系统脱髓鞘疾病。据信涉及促炎细胞因子γ干扰素（IFN-γ）的免疫机制在MS的发病机理中起重要作用。 IFN-β-1b已被引入作为MS的治疗药物，并被发现可减少临床加重的次数和严重程度。为了检查IFN-β-1b对髓鞘碱性蛋白（MBP）特异性和植物血凝素诱导的IFN-γ产生的影响，我们开发了一种细胞释放的捕获酶联免疫吸附测定（CRC-ELISA），该测定可快速测量自发性和抗原或有丝分裂原诱导的细胞IFN-γ产生。 CRC-ELISA记录了未经治疗的MS患者血液中显着的MBP特异性T细胞反应，这是通过IFN-γ产生来评估的。在接受IFN-β-1b治疗的MS患者中，这种反应受到抑制。本工作在体内证实了IFN-β-1b对MS中IFN-γ产生的体外抑制作用。此外，它为检测细胞因子提供了强大的新技术。

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