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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Specificity and Prevalence of Natural Bovine Antimannan Antibodies
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Specificity and Prevalence of Natural Bovine Antimannan Antibodies

机译：天然牛抗甘露聚糖抗体的特异性和流行性

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Immune responses to the carbohydrate components of microorganisms, mediated both by antibodies and by lectins, are an important part of host defense. In the present experiments, the specificity and presence of natural bovine antibodies against mannan, a common fungal antigen, were examined by enzyme-linked immunosorbent assay (ELISA), usingSaccharomyces cerevisiae mannan as an antigen. The results showed that all serum samples from animals of three age groups (newborn, calf, and adult) tested contained antimannan antibodies, and the titer of these antibodies increased significantly in adults. However, titers among individual adult cattle differed widely. Inhibition assays showed that yeast mannan was the strongest inhibitor.d-Mannose exhibited only a minor inhibitory effect at high concentrations. This suggests that most of these antibodies recognize an oligosaccharide-based epitope(s) different from those recognized by lectins. Cattle possess three serum C-type lectins (collectins) capable of recognizing mannan in a calcium-dependent manner. Addition of EDTA to the reaction did not reduce antibody binding, suggesting that the binding of these antibodies to mannan was not affected by the presence of collectin. The antibodies purified from either calf or adult serum by mannan-Sepharose affinity chromatography consisted of mainly immunoglobulin G (IgG) and a smaller amount of IgM. IgG1 was shown to be the dominant antimannan IgG isotype by isotype-specific ELISA. Together, these results demonstrate the production of natural antimannan antibodies in cattle in an age-dependent manner. These antibodies might be involved in defending the host against mannan-containing pathogens as a specific line of defense in conjunction with the innate response by lectins.
机译：由抗体和凝集素介导的对微生物碳水化合物成分的免疫反应是宿主防御的重要组成部分。在本实验中，以啤酒酵母（Saccharomyces cerevisiae）甘露聚糖为抗原，通过酶联免疫吸附法（ELISA）检测了天然牛抗甘露聚糖（一种常见的真菌抗原）的特异性和存在性。结果显示，来自三个年龄组（新生儿，小牛和成年）的动物的所有血清样品都含有抗甘露聚糖抗体，并且这些抗体的滴度在成年后显着增加。但是，成年个体牛的效价差异很大。抑制试验表明，酵母甘露聚糖是最强的抑制剂。高浓度的d-甘露糖只表现出较小的抑制作用。这表明大多数这些抗体识别与基于凝集素识别的那些不同的基于寡糖的抗原决定簇。牛具有三种能够以钙依赖方式识别甘露聚糖的血清C型凝集素（collectins）。向反应中加入EDTA不会降低抗体的结合，这表明这些抗体与甘露聚糖的结合不受收集素的存在的影响。通过甘露聚糖-琼脂糖亲和层析从牛犊或成年血清中纯化的抗体主要由免疫球蛋白G（IgG）和少量IgM组成。通过同种型特异性ELISA显示，IgG1是主要的抗甘露聚糖IgG同种型。总之，这些结果证明了牛中天然抗甘露聚糖抗体的产生具有年龄依赖性。这些抗体可能与凝集素的先天应答一起作为特定防御线参与防御宿主抵抗含甘露聚糖的病原体。

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