Identification of an IS711 Element Interrupting the wboA Gene of Brucella abortusVaccine Strain RB51 and a PCR Assay To Distinguish Strain RB51 from Other Brucella Species and Strains

Ramesh Vemulapalli; John R. McQuiston; Gerhardt G. Schurig; Nammalwar Sriranganathan; Shirley M. Halling; Stephen M. Boyle

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Identification of an IS711 Element Interrupting the wboA Gene of Brucella abortusVaccine Strain RB51 and a PCR Assay To Distinguish Strain RB51 from Other Brucella Species and Strains

机译：中断流产布鲁氏菌疫苗RB51的wboA基因的IS711元件的鉴定和区别其他布鲁氏菌属和菌株的RB51的PCR分析

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Brucella abortus vaccine strain RB51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. The genetic mutations that are responsible for the roughness and the attenuation of strain RB51 have not been identified until now. Also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain RB51 from its parent strain 2308 is available. In the present study, we demonstrate that thewboA gene encoding a glycosyltransferase, an enzyme essential for the synthesis of O antigen, is disrupted by an IS711 element in B. abortus vaccine strain RB51. Exploiting this feature, we developed a PCR assay that distinguishes strain RB51 from all other Brucella species and strains tested.

机译：流产布鲁氏菌疫苗菌株RB51是一种自然稳定的减毒粗糙突变体，衍生自有毒力菌株2308。到目前为止，尚未确定导致RB51菌株粗糙和减毒的遗传突变。而且，除了基于脉冲场凝胶电泳的测定法之外，没有其他简单的方法可以将菌株RB51与其亲本菌株2308区分。在本研究中，我们证明了 wboA 基因编码糖基转移酶（一种合成O抗原所必需的酶）被中的IS 711 元件破坏B．流产疫苗菌株RB51。利用此功能，我们开发了一种PCR检测方法，可将RB51菌株与所有其他 Brucella 物种和测试菌株区分开。 展开▼

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《Clinical and diagnostic laboratory immunology》 |1999年第5期|共5页

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Ramesh Vemulapalli; John R. McQuiston; Gerhardt G. Schurig; Nammalwar Sriranganathan; Shirley M. Halling; Stephen M. Boyle;
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1. Multiplex PCR Assay for the Identification and Differentiation of all Brucella Species and the Vaccine Strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1 [J] . Clara M. Marín, David García-Yoldi, Ignacio López-Go?i, Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2006,第4期

机译：用于鉴定和鉴别所有布鲁氏菌种和疫苗株的多重PCR分析法流产布鲁氏菌S19和RB51和melitensis Rev1

2. Immune Responses and Protection against Experimental Challenge after Vaccination of Bison with Brucella abortus Strain RB51 or RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes [J] . S. C. Olsen, S. M. Boyle, G. G. Schurig, Clinical and vaccine immunology: CVI . 2009,第4期

机译：用过表达超氧化物歧化酶和糖基转移酶基因的流产布鲁氏菌菌株RB51或RB51接种野牛后的免疫应答和对实验挑战的防护

3. Booster vaccination with safe, modified, live-attenuated mutants of Brucella abortus strain RB51 vaccine confers protective immunity against virulent strains of B. abortus and Brucella canis in BALB/c mice [J] . Quang Lam Truong, Youngjae Cho, Kiju Kim, Microbiology . 2015,第11期

机译：用安全，改良的减毒布鲁氏杆菌流产RB51疫苗的活突变株加强免疫接种，可对BALB / c小鼠的流产布鲁氏菌和犬布鲁氏菌的强毒株提供保护性免疫

4. Safety of Brucella abortus and RB51 and strain 19 vaccines in coyotes (Canis latrans) [C] . Donald S. Davis, Thomas J. Roffe, Phil H. Elzer Annual Meeting of the United States Animal Health Association . 2000

机译：Brucella abortus和Rb51和RB51和菌株19疫苗的安全性（Canis Latrans）

5. Genetic and biochemical characterization of Brucella abortus strain RB51. [D] . Jensen, Allen Elroy. 1995

机译：布鲁氏菌流产RB51菌株的遗传和生化特性。

6. Note: Identification of an IS711 Element Interrupting the wboA Gene of Brucella abortus Vaccine Strain RB51 and a PCR Assay To Distinguish Strain RB51 from Other Brucella Species and Strains [O] . Ramesh Vemulapalli, John R. McQuiston, Gerhardt G. Schurig, 1999

机译：注意：IS711元素的标识中断流产布鲁氏菌的wboA基因 RB51疫苗株和PCR鉴定RB51株其他布鲁切拉菌种和菌株

7. Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51 [O] . B J Bricker, S M Halling 1995

机译：Brucella Abortus疫苗菌株S19和RB51分化的Brucella AMOS PCR测定的增强

1. NLRP3炎症小体在牛种布鲁氏菌2308和RB51侵染人巨噬细胞THP-1过程中作用的初步研究 [J] . 陈鹏博 ,张亚丽 ,王远志 . 石河子大学学报（自然科学版） . 2014,第004期

2. 布鲁氏菌WboA基因的原核表达与鉴定 [J] . 张艳 ,陈创夫 ,张辉 . 石河子大学学报（自然科学版） . 2011,第002期

3. 用流产布氏杆菌菌株RB51防制牛布氏杆菌病 [J] . 李凯年 . 畜牧兽医科技信息 . 1997,第007期

4. 流产布鲁氏杆菌RB51疫苗对性成熟公牛和怀孕青年母牛的生物安全性试验 [J] . 钟发刚 ,王新华 . 中国奶牛 . 2001,第003期

5. 布鲁氏菌疫苗株S2全基因组测序及牛羊布鲁氏菌比较基因组学研究 [J] . 红梅 ,冯陈晨 ,岳建伟 . 中国人兽共患病学报 . 2014,第011期

6. 流产布鲁氏菌疫苗候选菌株RB6种子批的建立 [C] . 王真欧 ,齐星 ,武莉莉 . 中国畜牧兽医学会2011学术年会 . 2011

7. 布鲁氏菌RB51全基因组序列的初步分析 [A] . 马卧龙 . 2014

1. 流产布鲁氏菌免疫标记重组菌株与其在制备疫苗中的应用 [P] . 中国专利： CN101629151B . 2011.08.03

2. 流产布鲁氏菌免疫标记重组菌株与其在制备疫苗中的应用 [P] . 中国专利： CN101629151A . 2010-01-20

3. 8kDa antigen of Brucella abortus strain RB51, a method for purification thereof and a method for detecting antibody utilizing antigen of Brucella abortus strain RB51 [P] . 外国专利： KR100424137B1 . 2004-03-24

机译：流产布鲁氏菌菌株RB51的8kDa抗原，其纯化方法和利用抗体检测抗原的方法

4. 51 8 51 8kDa antigen of Brucella abortus strain RB51 a method for purification thereof and a method for detecting antibody utilizing antigen of Brucella abortus strain RB51 [P] . 外国专利： KR20020038844A . 2002-05-24

机译：流产布鲁氏菌菌株RB51的51 8 51 8kDa抗原及其纯化方法和利用该抗体的抗体检测方法

5. Primer set for Brucella canis strain-specific identification and method for detecting Brucella canis strain using the same [P] . 外国专利： KR101437389B1 . 2014-09-04

机译：用于布鲁氏菌菌株特异性鉴定的引物组以及使用该引物组检测犬布鲁氏菌菌株的方法

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Identification of an IS711 Element Interrupting the wboA Gene of Brucella abortusVaccine Strain RB51 and a PCR Assay To Distinguish Strain RB51 from Other Brucella Species and Strains

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