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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Giant Syncytia and Virus-Like Particles in Ovarian Carcinoma Cells Isolated from Ascites Fluid
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Giant Syncytia and Virus-Like Particles in Ovarian Carcinoma Cells Isolated from Ascites Fluid

机译：从腹水分离的卵巢癌细胞中的巨大合胞体和病毒样颗粒

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Ovarian cancer cells were isolated from ascites fluid of 30 different patients diagnosed with cystadenocarcinoma of ovaries. Large colonies of malignant ASC cells were observed during the first week of cell growth in vitro. Colony formation was followed by fusion of cells and formation of large multinucleated and highly vacuolated syncytia. In contrast, cells isolated from the ascites fluid produced by patients with benign mucinous cystadenoma of ovaries did not form syncytia. Nonmalignant Brenner tumor cells, isolated from the ascites fluid, also did not form syncytia. Syncytia, but not the nonmalignant tumor cells, were immunofluorescence stained with an anti-human immunodeficiency virus type 1 (HIV-1) gp120 monoclonal antibody (MAb) and MAb RAK-BrI. Both MAbs recognized cancer-associated antigens RAK (for Rakowicz markers) p120, p42, and p25. Exposure of ASC cells to either the anti-HIV-1 gp120 MAb or MAb RAK-BrI inhibited syncytium formation. PCR with HIV-1 Env-derived primers revealed DNA sequences with over 90% homology to HIV-1 gp41 in syncytia and in ovarian cancer cells but not in normal ovary cells. Electron microscopic analysis revealed viral particles, hexagonal in shape (90 nm in diameter), with a dense central core surrounded by an inner translucent capsid and dense outer shell with projections. Negative staining detected membrane-covered particles (100 to 110 nm in diameter) in the cell culture medium. Incubation of normal breast cells with viral particles resulted in drastic morphological changes and syncytium formation by the transformed breast cells. The cytopathic effects of the identified virus resembled those of spumaviruses, which, in addition to their epitopic and genetic homology to HIV-1, might suggest a common phylogeny.
机译：从30名诊断为卵巢囊腺癌的不同患者的腹水中分离出卵巢癌细胞。在体外细胞生长的第一周内观察到了大的ASC细胞集落。集落形成之后，细胞融合并形成大的多核和高度空泡的合胞体。相反，从卵巢良性粘液性囊腺瘤患者产生的腹水中分离出的细胞没有形成合胞体。从腹水中分离出的非恶性Brenner肿瘤细胞也未形成合胞体。用抗人免疫缺陷病毒1型（HIV-1）gp120单克隆抗体（MAb）和MAb RAK-BrI对合胞体而非非恶性肿瘤细胞进行免疫荧光染色。两种单克隆抗体均识别与癌症相关的抗原RAK（用于Rakowicz标记）p120，p42和p25。将ASC细胞暴露于抗HIV-1 gp120 MAb或MAb RAK-BrI会抑制合胞体的形成。使用HIV-1 Env衍生引物进行的PCR显示，合胞体和卵巢癌细胞中与HIV-1 gp41的DNA序列具有90％以上的同源性，而正常卵巢细胞中则没有。电子显微镜分析显示病毒颗粒呈六边形形状（直径90 nm），具有密集的中心核，内部的半透明衣壳和密集的带有突起的外壳包围。阴性染色检测到细胞培养基中被膜覆盖的颗粒（直径为100至110 nm）。正常乳腺细胞与病毒颗粒一起孵育会导致急剧的形态变化和转化的乳腺细胞形成合胞体。鉴定出的病毒的细胞病变作用类似于spumaviruses，除了其与HIV-1的表位和遗传同源性外，还可能提示其共同的系统发育。

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