...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Immunophenotyping of Peripheral Blood Lymphocytes in Saudi Men
【24h】

Immunophenotyping of Peripheral Blood Lymphocytes in Saudi Men

机译：沙特阿拉伯男子外周血淋巴细胞的免疫分型

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Flow cytometry is an important tool for the diagnosis and follow-up of immunodeficiency patients, as well as for pateints with leukemia and lymphoma. Lymphocytes and their subsets show variations with race. The aim of this study was to establish reference ranges for lymphocytes and their subsets in an Saudi adult population by using flow cytometry. Blood samples obtained from 209 healthy Saudi men were used for this study. All blood donors were between 18 and 44 years old. Lymphocytes and their subsets were analyzed by flow cytometry, and the absolute and percentage values were calculated. We investigated the expression of T-cell markers (CD3, CD4, and CD8), B cells (CD19), and natural killer cells (CD16 and CD56). The absolute and percent values of each cell subset were compared with published data from different populations by using the Student t test. Reference ranges, each expressed as the mean ± the standard deviation, were as follows: leukocytes (6,335 ± 1759), total lymphocytes (2,224 ± 717), CD3 cells (1,618 ± 547), CD4 cells (869 ± 310), CD8 cells (615 ± 278), CD19 cells (230 ± 130), and CD3-CD16+/CD56+ cells (262 ± 178). The CD4/CD8 ratio was 1.6 ± 0.7. Our results for B cells, CD4 cells, and CD8 cells and for the CD4/CD8 ratio fell in between the reported results for Ethiopian and Dutch subjects. Our results were also different from previously reported findings in an Saudi adult population that showed no increase in CD8 T cells. We thus establish here the reference ranges for lymphocytes and their subsets in a large cohort of Saudi men. The CD8 cell count was not abnormally high, as previously reported, and fell in between previous results obtained for African and European populations.
机译：流式细胞仪是诊断和随访免疫缺陷患者以及患有白血病和淋巴瘤的患者的重要工具。淋巴细胞及其子集显示种族差异。本研究的目的是通过流式细胞术建立沙特成年人群中淋巴细胞及其亚群的参考范围。本研究使用了从209名健康的沙特阿拉伯男子那里获得的血液样本。所有的献血者年龄在18至44岁之间。通过流式细胞术分析淋巴细胞及其亚群，并计算绝对值和百分比值。我们研究了T细胞标记（CD3，CD4和CD8），B细胞（CD19）和自然杀伤细胞（CD16和CD56）的表达。使用Student t 检验，将每个单元格子集的绝对值和百分比值与来自不同人群的已发布数据进行比较。参考范围分别表示为平均值±标准差，如下：白细胞（6,335±1759），总淋巴细胞（2,224±717），CD3细胞（1,618±547），CD4细胞（869±310），CD8细胞（615±278），CD19细胞（230±130）和CD3-CD16 + / CD56 +细胞（262±178）。 CD4 / CD8比为1.6±0.7。我们的B细胞，CD4细胞和CD8细胞结果以及CD4 / CD8比值的结果介于埃塞俄比亚人和荷兰人报告的结果之间。我们的结果也不同于先前报道的在沙特成年人群中发现的CD8 T细胞没有增加的发现。因此，我们在这里建立了大量沙特男性队列中淋巴细胞及其亚群的参考范围。如先前报道，CD8细胞计数并非异常高，介于非洲和欧洲人群先前获得的结果之间。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号