Extracellular proteinase activity of Cryptococcus neoformans.

L C Chen; E S Blank; A Casadevall

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Extracellular proteinase activity of Cryptococcus neoformans.

机译：新型隐球菌的胞外蛋白酶活性。

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Extracellular proteinase activity was studied for eight strains of Cryptococcus neoformans var. neoformans and two strains of Cryptococcus neoformans var. gattii. Proteinase activity was measured by protein agar clearance, azoalbumin hydrolysis, gelatin liquefaction, and protein substrate polyacrylamide gel electrophoresis. All strains of C. neoformans produced extracellular proteolytic activity. Maximal extracellular proteinase activity in supernatants of C. neoformans cultures was associated with late logarithmic- and stationary-phase cultures. C. neoformans was able to utilize murine immunoglobulin G1, bovine immunoglobulin G, and human complement factor 5 for growth in media containing these proteins as the sole sources of carbon and nitrogen, suggesting a capacity to degrade immunologically important proteins. Protein substrate polyacrylamide gel electrophoresis revealed several bands with proteolytic activity at apparent molecular masses of 200, 100, and 50 kDa. The results confirm the existence of extracellular proteinase activity for C. neoformans.

机译：研究了八种新隐球菌变种的细胞外蛋白酶活性。新型和两种新隐球菌菌株。加蒂。通过蛋白质琼脂清除率，偶氮白蛋白水解，明胶液化和蛋白质底物聚丙烯酰胺凝胶电泳来测量蛋白酶活性。所有新孢梭菌菌株均产生细胞外蛋白水解活性。新孢子虫培养物上清液中最大的细胞外蛋白酶活性与对数后期和固定期培养有关。新型梭状芽胞杆菌能够利用鼠类免疫球蛋白G1，牛免疫球蛋白G和人补体因子5在含有这些蛋白质作为唯一碳和氮源的培养基中生长，这表明其具有降解免疫学重要蛋白质的能力。蛋白底物聚丙烯酰胺凝胶电泳显示了几个具有蛋白水解活性的条带，表观分子量分别为200、100和50 kDa。结果证实了新孢梭菌存在细胞外蛋白酶活性。

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《Clinical and diagnostic laboratory immunology》 |1996年第5期|共5页
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L C Chen; E S Blank; A Casadevall;
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1. Extracellular phospholipase activity is a virulence factor for Cryptococcus neoformans. [J] . Cox GM, McDade HC, Chen SC, Molecular Microbiology . 2001,第1期

机译：细胞外磷脂酶活性是新隐球菌的毒力因子。
2. Inhibition of HIV entry by extracellular glucuronoxylomannan of Cryptococcus neoformans. [J] . Yi H. A., Panepinto J. C., Jacobs A. Microbial Pathogenesis . 2012,第1期

机译：新型隐球菌的胞外葡糖醛酸氧甘露聚糖抑制HIV进入。
3. Sec6-dependent sorting of fungal extracellular exosomes and laccase of Cryptococcus neoformans. [J] . Panepinto J, Komperda K, Frases S, Molecular Microbiology . 2009,第5期

机译：Sec6依赖的真菌隐球菌的真菌细胞外泌体和漆酶的排序。
4. Screening, Identification and Optimization of Extracellular Lipase Production of Yeast (Cryptococcus flavescens) Isolated from a Tree Canopy Fern in the Mount Makiling Forest Reserve, Philippines [C] . Francisco Elegado, Charisse Leanne Legaspi, Joseph Martin Paet, International Conference on Biosciences and Medical Engineering . 2019

机译：菲律宾山上雨林森林储备的树冠蕨类植物酵母（Cryptococcus Flavescens）细胞外脂肪酶生产的筛选，鉴定和优化
5. Investigation of higher plants for potential antiinfective agents: Part I. Isolation and antimicrobial activity of fusaric acid and dehydrofusaric acid from Fusarium oxysporum and Part II. Isolation and structure elucidation of components of Baphia nitida Lodd and Part III. Evaluation of higher plant constituents in a novel assay for the potential inhibition of phenoloxidase in Cryptococcus neoformans. [D] . Hatch, Deanna Mari. 1995

机译：高等植物潜在抗感染药的研究：第一部分。尖孢镰刀菌中富马酸和脱氢富马酸的分离和抑菌活性，第二部分。楠（Baphia nitida Lodd）和第三部分的成分的分离和结构解析。在新型测定中评估新型隐球菌中酚氧化酶的潜在抑制作用。
6. Extracellular proteinase activity of Cryptococcus neoformans. [O] . L C Chen, E S Blank, A Casadevall 1996

机译：新型隐球菌的胞外蛋白酶活性。
7. Quimiotipagem do Cryptococcus neoformans. Revisão da literatura. Novos dados epidemiológicos sobre a criptococose. Nossa experiência com o emprego do meio de C.G.B. no estudo daquela levedura Biotyping of Cryptococcus neoformans. Review of the literature. New epidemiologic informations about cryptococcosis. Our experience with the utilization of C.G.B medium in this yeast [O] . Natalina Takahashi de Melo, Carlos da Silva Lacaz, Cecília Eugênia Charbel, 1993

机译：新型隐球菌的化学分型。文献修订。关于隐球菌病的新流行病学数据。我们使用C.G.B.的经验在研究酵母新隐球菌的生物型。文献回顾。有关隐球菌病的新流行病学信息。我们在这种酵母中利用C.G.B培养基的经验

Extracellular proteinase activity of Cryptococcus neoformans.

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