...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Peptide sequences of glycoprotein G-2 discriminate between herpes simplex virus type 2 (HSV-2) and HSV-1 antibodies.
【24h】

Peptide sequences of glycoprotein G-2 discriminate between herpes simplex virus type 2 (HSV-2) and HSV-1 antibodies.

机译：糖蛋白G-2的肽序列可区分2型单纯疱疹病毒（HSV-2）和HSV-1抗体。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The complete herpes simplex virus type 2 envelope glycoprotein G was represented by overlapping synthetic peptides. Herpes simplex virus type 2-specific human seroreactivities were mainly seen against three peptides, peptides G2-64, G2-69, and G2-70, located in the C-terminal part of glycoprotein G. This is, interestingly, a region which has strong homology between herpes simplex virus types 1 and 2. G2-69 was the most herpes simplex virus type 2-specific peptide, reacting with 93% (13 of 14) of herpes simplex virus type 2 immunoglobulin G-positive human serum samples (n = 14) and none of the type 2 negative serum samples (n = 16) tested. The epitope of peptide G2-69 was mapped to the amino acid sequence RYAHPS, and the antibody binding to G2-64 could be increased by chemical oxidation of the peptide. The three reactive peptides were used, alone or combined, together with herpes simplex virus type 2 antigen in an attempt to discriminate anti-herpes simplex virus type 2 from anti-herpes simplex virus type 1 immunoglobulin G. Inclusion of the herpes simplex virus type 2 glycoprotein G peptides increased the specificity compared with the use of glycoprotein G alone. Combinations of peptides with whole glycoprotein also showed a greater discriminative capacity than single peptides. We conclude that these synthetic glycoprotein G peptides may be useful for herpes simplex virus type 2 serology based on peptides or combinations of peptides and antigens. This is the first study describing the possibility of discriminative herpes simplex virus serology by using synthetic peptides combined with small amounts of whole glycoprotein G.
机译：完整的单纯疱疹病毒2型包膜糖蛋白G以重叠的合成肽表示。单纯疱疹病毒2型特异性人类血清反应性主要针对位于糖蛋白G C端的三种肽G2-64，G2-69和G2-70。 1型和2型单纯疱疹病毒之间具有很强的同源性。G2-69是2型疱疹最特异性的肽，与93％（14个中的13个）2型单纯疱疹病毒免疫球蛋白G阳性人血清反应（n = 14），没有任何2型阴性血清样本（n = 16）被测试。肽G2-69的表位被定位到氨基酸序列RYAHPS，并且通过肽的化学氧化可以增加与G2-64的抗体结合。三种反应性肽与2型单纯疱疹病毒抗原一起单独使用或组合使用，试图将2型抗单纯疱疹病毒与1型单纯疱疹病毒免疫球蛋白G区分开。2型单纯疱疹病毒包括在内与单独使用糖蛋白G相比，糖蛋白G肽增加了特异性。肽与完整糖蛋白的组合也显示出比单个肽更大的判别能力。我们得出结论，这些合成的糖蛋白G肽可能对基于肽或肽与抗原组合的2型单纯疱疹病毒血清学有用。这是第一项描述通过使用合成肽与少量完整糖蛋白G结合而鉴别出单纯疱疹病毒血清学的可能性的研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号