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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.
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Humoral immune response against lipopolysaccharide and cytoplasmic proteins of Brucella abortus in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9.

机译：接种流产双歧杆菌S19或实验性感染肠球菌耶尔森氏菌血清型0：9的牛对布鲁氏菌流产的脂多糖和胞质蛋白的体液免疫应答。

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摘要

The humoral immune responses against three different antigens of Brucella abortus were monitored by enzyme-linked immunosorbent assay in cattle vaccinated with B. abortus S19 or experimentally infected with Yersinia enterocolitica serotype 0:9. Immunoglobulin G (IgG) and IgM responses against (i) B. abortus lipopolysaccharide (LPS), (ii) total cytoplasmic proteins depleted of LPS (LPS-free CYT), and (iii) B. abortus 18-kDa cytoplasmic protein were measured. Vaccinated animals and Yersinia-infected animals developed high anti-LPS IgM and IgG titers, which overlapped with those obtained with sera from B. abortus 544-infected animals used as positive controls. In contrast, only a slight or negative IgG and IgM response against LPS-free CYT and the 18-kDa protein was detected in vaccinated or Yersinia-infected cattle, although its levels were always significantly lower than those of B. abortus 544-infected animals. These data indicate that cytoplasmic proteins of B. abortus could be useful for the differential diagnosis of bovine brucellosis.
机译：通过酶联免疫吸附测定法监测了接种流产双歧杆菌S19或实验性感染肠球菌耶尔森氏菌血清型0：9的牛对布鲁氏菌流产的三种不同抗原的体液免疫反应。测量了针对（i）流产双歧杆菌脂多糖（LPS），（ii）消耗了LPS的总胞质蛋白（不含LPS CYT）和（iii）流产双歧杆菌18-kDa细胞质的免疫球蛋白G（IgG）和IgM反应。 。接种疫苗的动物和感染耶尔森氏菌的动物具有很高的抗-LPS IgM和IgG滴度，这与用流产双歧杆菌感染544只动物的血清作为阳性对照的结果相吻合。相反，在疫苗接种或感染耶尔森氏菌的牛中，仅检测到针对无LPS的CYT和18-kDa蛋白的轻度或阴性IgG和IgM反应，尽管其水平始终明显低于流产双歧杆菌544感染的动物。这些数据表明流产双歧杆菌的胞质蛋白可用于牛布鲁氏菌病的鉴别诊断。

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