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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Use of Egg Yolk-Derived Immunoglobulin as an Alternative to Antibiotic Treatment for Control of Helicobacter pylori Infection
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Use of Egg Yolk-Derived Immunoglobulin as an Alternative to Antibiotic Treatment for Control of Helicobacter pylori Infection

机译：使用蛋黄衍生的免疫球蛋白替代抗生素治疗幽门螺杆菌感染

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The present study evaluated the potential use of immunoglobulin prepared from the egg yolk of hens immunized with Helicobacter pylori (immunoglobulin Y [IgY]-Hp) in the treatment of H. pylori infections. The purity of our purified IgY-Hp was 91.3%, with a yield of 9.4 mg of IgY per ml of egg yolk. The titer for IgY-Hp was 16 times higher than that for IgY in egg yolk from nonimmunized hens, and IgY-Hp significantly inhibited the growth and urease activity of H. pylori in vitro. Bacterial adhesion on AGS cells was definitely reduced by preincubation of both H. pylori (108 CFU/ml) and 10 mg of IgY-Hp/ml. In Mongolian gerbil models, IgY-Hp decreased H. pylori-induced gastric mucosal injury as determined by the degree of lymphocyte and neutrophil infiltration. Therefore, in this experimental model, H. pylori-associated gastritis could be successfully treated by orally administered IgY-Hp. The immunological activity of IgY-Hp stayed active at 60°C for 10 min, suggesting that pasteurization can be applied to sterilize the product. Fortification of food products with this immunoglobulin would significantly decrease the H. pylori infection. In conclusion, the IgY-Hp obtained from hens immunized by H. pylori could provide a novel alternative approach to treatment of H. pylori infection.
机译：本研究评估了用幽门螺杆菌免疫的母鸡的蛋黄（免疫球蛋白Y [IgY] -Hp）制备的免疫球蛋白在治疗 H中的潜在用途。幽门螺杆菌感染。我们纯化的IgY-Hp的纯度为91.3％，每毫升蛋黄的产量为9.4 mg IgY。在未免疫母鸡的蛋黄中，IgY-Hp的效价是IgY的效价的16倍，并且IgY-Hp显着抑制 H的生长和脲酶活性。幽门螺杆菌两种H均经过预温育，肯定会降低AGS细胞上的细菌粘附。幽门螺杆菌（10 8 CFU / ml）和10 mg IgY-Hp / ml。在蒙古沙鼠模型中，IgY-Hp降低了 H。根据淋巴细胞和中性粒细胞浸润的程度确定幽门螺杆菌引起的胃粘膜损伤。因此，在该实验模型中， H。口服IgY-Hp可成功治疗幽门螺杆菌相关性胃炎。 IgY-Hp的免疫活性在60°C保持10分钟的活性，这表明可以应用巴氏灭菌法对产品进行灭菌。用这种免疫球蛋白强化食品将大大降低 H。幽门螺杆菌感染。总之，从通过 H免疫的母鸡获得的IgY-Hp。幽门螺杆菌可以为 H提​​供一种新颖的替代治疗方法。幽门螺杆菌感染。

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