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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Longitudinal Profile of Immunoglobulin G (IgG), IgM, and IgA Antibodies against the Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein in Patients with Pneumonia Due to the SARS Coronavirus
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Longitudinal Profile of Immunoglobulin G (IgG), IgM, and IgA Antibodies against the Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein in Patients with Pneumonia Due to the SARS Coronavirus

机译：SARS冠状病毒引起的肺炎患者中针对严重急性呼吸系统综合症（SARS）冠状病毒核蛋白的免疫球蛋白G（IgG），IgM和IgA抗体的纵向概况

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By using a recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein-based enzyme-linked immunosorbent assay (ELISA) and serum specimens serially collected (from day 0 to day 240 after symptom onset) from patients with pneumonia due to SARS-CoV, we analyzed the longitudinal profiles of immunoglobulin G (IgG), IgM, and IgA antibodies against the SARS-CoV nucleocapsid protein in patients with pneumonia due to SARS-CoV. For IgG, the median optical density at 450 nm (OD450) turned positive at day 17 and a biphasic response was observed. At day 240, all patients were still positive for anti-nucleocapsid protein IgG antibody. For IgM, the median OD450 turned positive at day 20.5, peaked at about day 80, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgM antibody. For IgA, the median OD450 turned positive at day 17, peaked at about day 50, and fell to below the baseline level at about day 180. At day 240, 36% of the patients were still positive for anti-nucleocapsid protein IgA antibody. The time of seroconversion detected by the recombinant SARS-CoV nucleocapsid protein-based ELISA and that detected by indirect immunofluorescence assay were similar. The median times of seroconversion for IgG, IgM, and IgA detected by the indirect immunofluorescence assay were 17 days (17 days by ELISA), 16.5 days (20.5 days by ELISA), and 17.5 days (17 days by ELISA), respectively, after disease onset. One, four, and one of the six patients who died did not produce any IgG, IgM, and IgA antibodies against the nucleocapsid protein of SARS-CoV, respectively, although these antibodies were detected in all six patients by the indirect immunofluorescence assay. Further studies should be performed to see whether SARS-CoV nucleocapsid protein antibody positivity has any prognostic significance.
机译：通过使用重组严重急性呼吸系统综合症冠状病毒（SARS-CoV）基于核衣壳蛋白的酶联免疫吸附测定（ELISA）和血清样本（从症状发作后第0天到第240天）连续收集因SARS-引起的肺炎患者CoV，我们分析了由于SARS-CoV引起的肺炎患者中针对SARS-CoV核​​衣壳蛋白的免疫球蛋白G（IgG），IgM和IgA抗体的纵向概况。对于IgG，在第17天时在450 nm处的中值光密度（OD 450 ）变为正，并观察到双相反应。在第240天，所有患者的抗核衣壳蛋白IgG抗体仍为阳性。对于IgM，中值OD 450 在第20.5天转为阳性，在第80天达到峰值，在第180天降至基线水平以下。在第240天，仍有36％的患者为阳性用于抗核衣壳蛋白IgM抗体。对于IgA，中值OD 450 在第17天转为阳性，在第50天达到峰值，在第180天降至基线水平以下。在第240天，仍有36％的患者为阳性用于抗核衣壳蛋白IgA抗体。基于重组SARS-CoV核​​衣壳蛋白的ELISA检测的血清转化时间与间接免疫荧光检测的血清转化时间相似。间接免疫荧光法检测到的IgG，IgM和IgA血清转化的中位时间分别为17天（ELISA法为17天），16.5天（ELISA法为20.5天）和17.5天（ELISA法为17天）。疾病发作。死亡的六名患者中的一，四和一没有分别产生针对SARS-CoV核​​衣壳蛋白的IgG，IgM和IgA抗体，尽管通过间接免疫荧光分析在所有六名患者中均检测到了这些抗体。应进行进一步研究，以查看SARS-CoV核​​衣壳蛋白抗体阳性是否对预后具有重要意义。

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