...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Leukotriene B4 Receptor (BLT-1) Modulates Neutrophil Influx into the Peritoneum but Not the Lung and Liver during Surgically Induced Bacterial Peritonitis in Mice
【24h】

Leukotriene B4 Receptor (BLT-1) Modulates Neutrophil Influx into the Peritoneum but Not the Lung and Liver during Surgically Induced Bacterial Peritonitis in Mice

机译：白三烯B4受体（BLT-1）调节小鼠手术性细菌性腹膜炎期间嗜中性粒细胞流入腹膜，但不影响肺和肝。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Leukotriene B4 (LTB4) is a rapidly synthesized, early neutrophil chemoattractant that signals via its cell surface receptor, BLT-1, to attract and activate neutrophils during peritonitis. BLT-1-deficient (BLT-1?/?) mice were used to determine the effects of LTB4 on neutrophil migration and activation, bacterial levels, and survival after cecal ligation and puncture (CLP). Male BLT-1?/? or wild-type (WT) BALB/c mice underwent CLP. Tissues were harvested for determination of levels of bacteria, myeloperoxidase (MPO), LTB4, macrophage inflammatory protein 2 (MIP-2), and neutrophil (polymorphonuclear leukocyte [PMN]) numbers at 4 and 18 h after CLP. PMN activation was determined by an assessment of phagocytosis ability and CD11b expression. Survival was also determined. BLT-1?/? mice had decreased numbers of PMNs in the peritoneum at both 4 and 18 h after CLP but increased numbers of PMNs in the blood at 18 h compared with WT mice. Liver and lung MPO levels were significantly higher in BLT-1?/? mice at both 4 and 18 h after CLP, with increased bacterial levels in the blood, the liver, and peritoneal fluid at 4 h. Bacterial levels remained higher in peritoneal fluid at 18 h, but blood and liver bacterial levels at 18 h were not different from levels at 4 h. PMN phagocytosis and CD11b levels were decreased in BLT-1?/? mice. LTB4 levels were similar between the groups before and after CLP, but MIP-2 levels were decreased both locally and systemically in BLT-1?/? mice. Survival was significantly improved in BLT-1?/? mice (71%) compared with WT mice (14%) at 48 h post-CLP. Thus, LTB4 modulates neutrophil migration into the mouse peritoneum, but not the lung or liver, after CLP. Despite higher bacterial and PMN levels at remote sites, there was increased survival in BLT-1?/? mice compared to WT mice. Decreased PMN activation may result in less remote organ dysfunction and improved survival.
机译：白三烯B 4 （LTB 4 ）是一种快速合成的早期中性粒细胞趋化因子，通过其细胞表面受体BLT-1发出信号，在腹膜炎期间吸引并激活中性粒细胞。缺乏BLT-1（BLT-1 ？/？）的小鼠用于确定LTB 4 对盲肠中性粒细胞迁移和活化，细菌水平和存活的影响结扎和穿刺（CLP）。对雄性BLT-1 ？/？或野生型（WT）BALB / c小鼠进行CLP。收集组织以测定4、18 h细菌，髓过氧化物酶（MPO），LTB 4 ，巨噬细胞炎性蛋白2（MIP-2）和嗜中性白血球（多形核白细胞[PMN]）水平中电之后。通过评估吞噬能力和CD11b表达来确定PMN激活。还确定了存活率。与WT小鼠相比，BLT-1 α/β小鼠在CLP后4和18小时的腹膜PMN数量均减少，但18 h血液中PMN数量却增加。 CLP后4和18小时，BLT-1 ？/？小鼠的肝和肺MPO水平显着升高，而4小时时血液，肝脏和腹膜液中细菌水平升高。 18 h腹膜液中细菌水平仍然较高，但18 h时血液和肝细菌水平与4 h时无差异。 BLT-1 ？/？小鼠的PMN吞噬作用和CD11b水平降低。 CLP前后各组的LTB 4 水平相似，但BLT-1 α/β小鼠的局部和全身MIP-2水平均降低。在CLP后48小时，与WT小鼠（14％）相比，BLT-1 α/β小鼠的存活率显着提高（71％）。因此，LTB 4 会在CLP后调节中性粒细胞向小鼠腹膜的迁移，而不是肺或肝的迁移。尽管在偏远地区细菌和PMN含量较高，但与WT小鼠相比，BLT-1 α/β小鼠的存活率有所提高。 PMN激活减少可导致较少的远端器官功能障碍并改善生存率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号