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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Alterations in lymphocyte phenotype and function in children with shigellosis who develop complications.
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Alterations in lymphocyte phenotype and function in children with shigellosis who develop complications.

机译：发生并发症的志贺氏菌病患儿淋巴细胞表型和功能的改变。

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This study was designed to see whether alterations occur in peripheral blood mononuclear cell phenotype and function in children with Shigella dysenteriae 1 infection with complications (leukemoid reaction and/or hemolytic-uremic syndrome) and whether there are any alterations prior to the development of complications. The following groups of children (ages, 12 to 60 months) were compared: children without any infection (n = 51), children with uncomplicated shigellosis (n = 65), children admitted with complicated shigellosis (leukemoid reaction and/or hemolytic-uremic syndrome) (n = 29), and children with shigellosis who developed complications after enrollment (subsequently complicated shigellosis) (n = 12). Tests for the peripheral blood mononuclear cell phenotype (CD3, CD4, CD8, CD57 [corrected], CD20, and CD25), spontaneous proliferation, and the proliferative response to phytohemagglutinin, pokeweed mitogen, and the lipopolysaccharide of S. dysenteriae 1 were performed, as were skin tests for delayed-type hypersensitivity (DTH). Children who subsequently developed complications differed from other groups of children as follows: (i) the numbers of CD3+ and CD4+ cells were lower than in uninfected children (P < 0.05), (ii) the CD4/CD8 ratio was lower than in children with uncomplicated shigellosis (P < 0.05) and in uninfected children (P < 0.05), and (iii) the levels of spontaneous proliferation of peripheral blood mononuclear cells were higher and DTH responses were lower than those in children with uncomplicated shigellosis (P < 0.05 and P < 0.017, respectively). Children with complications differed by having (i) increased numbers of CD3- CD57- [corrected] CD20- cells (P < 0.05) compared with those in other groups of children and (ii) lower CD4/CD8 ratios (P < 0.05), higher levels of spontaneous proliferation (P < 0.05), and lower DTH responses (P = 0.005) than children with uncomplicated shigellosis. Three to five days after enrollment, the number of CD4+ cells increased in children who subsequently developed complications (P = 0.025), i.e., when they developed complications and at this time their CD4+ cell number was similar to that of other groups of children. Thus, lymphocyte phenotype and function are altered prior to the development of complications in children with shigellosis, and once complications develop, the pattern of alterations changes. Whether these alterations have a role in precipitating complications or whether they reflect early events underlying the development of complications remains to be elucidated.
机译：这项研究旨在观察患有痢疾志贺氏菌1型感染并发并发症（类白血病反应和/或溶血尿毒综合征）的儿童外周血单核细胞表型和功能是否发生变化，以及在发生并发症之前是否有任何变化。比较了以下几组儿童（12至60个月大）：无感染的儿童（n = 51），患有单纯性志贺氏菌病的儿童（n = 65），患有复杂志贺菌病的儿童（类白血病反应和/或溶血尿毒症）综合征（n = 29），以及患有志贺氏菌病的儿童，其入选后出现并发症（随后为复杂的志贺氏菌病）（n = 12）。进行了外周血单核细胞表型（CD3，CD4，CD8，CD57 [校正]，CD20和CD25），自发性增殖以及对植物血凝素，商陆有丝分裂原和痢疾链球菌1的脂多糖的增殖反应的测试，以及针对迟发型超敏反应（DTH）的皮肤测试。随后发生并发症的儿童与其他儿童组的差异如下：（i）CD3 +和CD4 +细胞的数量低于未感染儿童（P <0.05），（ii）CD4 / CD8的比例低于患有其他疾病的儿童。单纯性志贺氏菌病（P <0.05）和未感染儿童（P <0.05），以及（iii）外周血单个核细胞的自发增殖水平高于非单纯性志贺氏菌病儿童（P <0.05和P分别为<0.017）。有并发症的儿童与（i）与其他儿童组相比，CD3-CD57- [校正的] CD20-细胞数量增加（P <0.05），以及（ii）较低的CD4 / CD8比值（P <0.05），与单纯性志贺菌病患儿相比，自发增殖水平更高（P <0.05），DTH反应更低（P = 0.005）。入组后三到五天，随后出现并发症的儿童CD4 +细胞数量增加（P = 0.025），即当他们出现并发症时，此时其CD4 +细胞数量与其他组儿童相似。因此，志贺氏菌病患儿在发生并发症之前先改变淋巴细胞的表型和功能，一旦并发症发生，改变的方式就会改变。这些改变是否在促进并发症中起作用或它们是否反映了并发症发展的早期事件仍有待阐明。

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