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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Evidence for the presence of immunoglobulin E antibodies specific to the cell wall phosphomannoproteins of Candida albicans in patients with allergies.
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Evidence for the presence of immunoglobulin E antibodies specific to the cell wall phosphomannoproteins of Candida albicans in patients with allergies.

机译：有过敏反应的患者存在针对白色念珠菌细胞壁磷酸甘露糖蛋白的免疫球蛋白E抗体的证据。

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摘要

To determine the major antigenic component of Candida albicans against immunoglobulin E (IgE) antibodies in the sera of patients with allergies who were positive for IgE antibodies to C. albicans crude antigen in a CAP system, phosphomannoproteins (CAMP/A or CAMP/B for serotype A or B strain, respectively) and their acid-stable portions (CAMP-S/A or CAMP-S/B) were isolated from beta-mercaptoethanol (2-ME) extracts of C. albicans cells of serotypes A and B, and IgE antibodies against these components were compared with those against protein complex and enolase (CAE) fractions isolated from C. albicans cells. The dot blot test, which was used to detect IgE antibodies to the C. albicans antigens, showed that IgE antibodies to the 2-ME extract and phosphomannoprotein fractions were present in the sera of 98.0% (2-ME extract), 96.8% (CAMP/A), 93.2% (CAMP-S/A), 97.2% (CAMP/B), and 81.5% (CAMP-S/B) of the patients, whereas IgE antibodies to the protein complex and CAE fractions were found in the sera of 73.6 and 48.8% of the patients, respectively. The extent of IgE binding to the 2-ME extract and phosphomannoproteins was well correlated with the fluorescence intensities estimated with the CAP system. Furthermore, the results obtained from the inhibition experiment with the CAP system indicated that the binding of IgE antibodies to Candida antigens is strongly inhibited by the phosphomannoprotein fraction and is an indication that the serum of the patients contained IgE antibodies specific to the cell wall phosphomannoproteins of C. albicans. Finally, an initial chemical analysis indicated that the epitopes for IgE antibodies on the phosphomannoproteins is a carbohydrate portion, since the ability of CAMP/A to inhibit the binding of IgE antibodies to the homologous CAMP/A was destroyed after oxidation by sodium periodate but not after digestion with proteinase K.
机译：为确定在CAP系统中针对白色念珠菌粗抗原的IgE抗体呈阳性的过敏患者血清中白色念珠菌对免疫球蛋白E（IgE）抗体的主要抗原成分，使用磷酸甘露糖蛋白（CAMP / A或CAMP / B从A型和B型血清白念珠菌细胞的β-巯基乙醇（2-ME）提取物中分离出A型或B型血清型菌株及其酸稳定部分（CAMP-S / A或CAMP-S / B），将针对这些成分的IgE和IgE抗体与针对从白色念珠菌细胞分离的蛋白质复合物和烯醇酶（CAE）组分的抗体进行了比较。用于检测白色念珠菌抗原的IgE抗体的斑点印迹测试表明，针对2-ME提取物和磷酸甘露糖蛋白组分的IgE抗体在血清中的存在率为98.0％（2-ME提取物），96.8％（ CAMP / A），93.2％（CAMP-S / A），97.2％（CAMP / B）和81.5％（CAMP-S / B）的患者中发现了针对蛋白质复合物和CAE组分的IgE抗体患者的血清分别为73.6和48.8％。 IgE与2-ME提取物和磷酸甘露糖蛋白的结合程度与CAP系统估算的荧光强度密切相关。此外，从CAP系统的抑制实验获得的结果表明，磷酸甘露糖蛋白组分强烈抑制了IgE抗体与念珠菌抗原的结合，并且表明患者的血清中含有对细胞壁磷酸甘露糖蛋白特异的IgE抗体。白色念珠菌。最后，初步的化学分析表明磷酸甘露糖蛋白上IgE抗体的表位是碳水化合物部分，因为高碘酸钠氧化后破坏了CAMP / A抑制IgE抗体与同源CAMP / A结合的能力，但并未破坏用蛋白酶K消化后。

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