...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Detection of antibodies to Shigella lipopolysaccharide in urine after natural Shigella infection or vaccination.
【24h】

Detection of antibodies to Shigella lipopolysaccharide in urine after natural Shigella infection or vaccination.

机译：在自然志贺氏菌感染或接种疫苗后检测尿液中的志贺氏菌脂多糖抗体。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The purpose of the present study was to explore the possibility of detecting antibodies to Shigella sonnei lipopolysaccharide (LPS) in urine after infection or vaccination. Urinary immunoglobulin A (IgA) and IgG antibodies and specific IgA secretory protein against S. sonnei LPS were measured by enzyme-linked immunosorbent assay (ELISA), after adjustment for urine concentration. A significant antibody level was defined as one above a cutoff value calculated from the geometric mean + 2 standard deviations of urinary anti-S. sonnei LPS levels in 43 healthy hepatitis B vaccinees (controls). Of 11 culture-proven cases of S. sonnei shigellosis, at convalescence 9 (82%) had significantly elevated levels of urinary antibodies to the homologous LPS. The S. sonnei conjugate vaccine, composed of S. sonnei O-specific polysaccharide covalently bound to recombinant exoprotein A of Pseudomonas aeruginosa, elicited a significant urine IgA or IgG anti-LPS response in 60% (6 of 10), 56% (9 of 16) 43% (16 of 37), and 14% (3 of 21) of the volunteers at 2 weeks, 6 weeks, 6 months, and 12 months after vaccination, respectively. The specificity of the urine antibody response to S. sonnei LPS was documented by the total lack of response in subjects who received parenteral Shigella flexneri 2a-recombinant exoprotein A conjugate (69 urine samples) or meningoccal tetravalent control vaccines (4 urine samples). All the volunteers who lacked a significant response to S. sonnei LPS in serum also lacked such response in urine samples. Seventy-four percent of the volunteers with a significant IgA or IgG anti-LPS response in serum at convalescence or 14 days after vaccination showed a similar response in urine. The ratio of the titer of secretory protein bound to IgA anti-S. sonnei LPS in urine to that in serum was 303 times higher than the ratio of anti-S. sonnei LPS total IgA titer in urine to that in serum, indicating that the urine IgA is of secretory origin. These findings suggest the possible use of urinary Shigella LPS antibodies as markers of systemic and secretory immune responses after natural infection or vaccination. At this stage, because of its limited sensitivity, the detection by ELISA of Shigella LPS antibodies in urine cannot replace the same assay in serum as a definitive test in an individual with a negative result.
机译：本研究的目的是探讨在感染或接种疫苗后检测尿液中抗志贺氏菌脂多糖（LPS）抗体的可能性。调整尿液浓度后，通过酶联免疫吸附测定（ELISA）测量了针对S. sonnei LPS的尿液免疫球蛋白A（IgA）和IgG抗体以及特异性IgA分泌蛋白。显着抗体水平定义为高于从尿抗S的几何平均值+ 2个标准差计算得出的临界值之上的一个。 43种健康的乙型肝炎疫苗（对照）中的sonnei LPS水平。在11例经培养证实的沙门氏菌志贺氏菌病中，恢复期有9例（82％）的同源LPS尿抗体水平显着升高。由共价结合到铜绿假单胞菌的重组外蛋白A的S. sonnei O特异多糖组成的S. sonnei缀合物疫苗引起尿液中IgA或IgG的抗LPS应答显着，分别为60％（10之6），56％（9）分别在接种疫苗后2周，6周，6个月和12个月时，分别有43％（16名患者中的16名）和14％（21名患者中的3名）的志愿者。接受肠胃外弗氏志贺氏菌2a重组外蛋白A结合物（69份尿液样本）或脑膜炎四价对照疫苗（4份尿液样本）的受试者完全没有反应，证明了对链球菌LPS尿液抗体反应的特异性。所有对血清中的S. Sonnei LPS均无明显反应的志愿者在尿液样本中也缺乏这种反应。恢复期或接种疫苗后14天，血清中有明显IgA或IgG抗LPS反应的志愿者中有74％在尿液中表现出相似的反应。与IgA抗S结合的分泌蛋白的效价之比。尿液中的Sonnei LPS比血清中的Sonnei高出抗S比303倍。 sonnei LPS在尿液中的总IgA滴度要比在血清中的IgA滴度高，表明尿液IgA是分泌性来源。这些发现表明，在自然感染或接种疫苗后，可能将尿志贺氏菌LPS抗体用作全身和分泌性免疫反应的标志物。在此阶段，由于灵敏度有限，因此尿液中志贺氏菌LPS抗体的ELISA检测无法代替血清中作为确定性检测相同检测的阴性结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号