...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Clinical and diagnostic laboratory immunology >Novel enzyme-linked immunosorbent assays for the detection of anti-Fc gamma receptor autoantibodies.
【24h】

Novel enzyme-linked immunosorbent assays for the detection of anti-Fc gamma receptor autoantibodies.

机译：用于检测抗-Fcγ受体自身抗体的新型酶联免疫吸附测定。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is a substantial interest in the role of antineutrophil antibodies since Fc gamma receptors (Fc gamma Rs) have been identified as the target for the majority of such autoantibodies. Antineutrophil antibodies have long been detected by an indirect immunofluorescence technique. Following optimization of the flow cytometric method of detection, we developed three enzyme-linked immunosorbent assays (ELISAs), each specific for autoantibodies against one of the three classes of human Fc gamma R. Fc gamma RI and Fc gamma RII were purified from cultured cells, and Fc gamma RIII was produced as a recombinant molecule. These were then used as capture agents in the respective ELISAs. When applied in parallel to a sizeable group of patients with primary Sj?gren's syndrome, both methods established that anti-Fc gamma R autoantibodies were heterogeneous. This finding indicates that different populations of partly cross-reactive antibodies are detectable by these two methods.
机译：由于已经将Fcγ受体（FcγRs）鉴定为大多数此类自身抗体的靶标，因此抗中性粒细胞抗体的作用引起了极大的兴趣。长期以来，通过间接免疫荧光技术已检测到抗中性粒细胞抗体。在优化流式细胞仪检测方法之后，我们开发了三种酶联免疫吸附测定（ELISA），每种酶都针对针对三类人FcγR之一的自身抗体。从培养的细胞中纯化FcγRI和FcγRII。 ，FcγRIII作为重组分子生产。然后将它们用作各自的ELISA中的捕获剂。当与大量原发性干燥综合征患者并行使用时，两种方法均确定抗-FcγR自身抗体是异源的。该发现表明通过这两种方法可检测到不同的部分交叉反应抗体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号