• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Contrast media & molecular imaging >Neural Induction Potential and MRI of ADSCs Labeled Cationic Superparamagnetic Iron Oxide Nanoparticle In Vitro
【24h】

Neural Induction Potential and MRI of ADSCs Labeled Cationic Superparamagnetic Iron Oxide Nanoparticle In Vitro

机译：ADSCs标记的阳离子超顺磁性氧化铁纳米粒子的神经感应电位和MRI体外

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Magnetic resonance imaging (MRI) combined with contrast agents is believed to be useful for stem cell tracking in vivo, and the aim of this research was to investigate the biosafety and neural induction of SD rat-originated adipose derived stem cells (ADSCs) using cationic superparamagnetic iron oxide (SPIO) nanoparticle which was synthesized by the improved polyol method, in order to allow visualization using in vitro MRI. The scan protocols were performed with T2-mapping sequence; meanwhile, the ultrastructure of labeled cells was observed by transmission electron microscopy (TEM) while the iron content was measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES). After neural induction, nestin and NSE (neural markers) were obviously expressed. In vitro MRI showed that the cationic PEG/PEI-modified SPIO nanoparticles could achieve great relaxation performance and favourable longevity. And the ICP-AES quantified the lowest iron content that could be detected by MRI as 1.56~1.8 pg/cell. This study showed that the cationic SPIO could be directly used to label ADSCs, which could then inductively differentiate into nerve and be imaged by in vitro MRI, which would exhibit important guiding significance for the further in vivo MRI towards animal models with neurodegenerative disorders.
机译：磁共振成像（MRI）与造影剂的结合被认为可用于体内干细胞的追踪，这项研究的目的是研究使用阳离子阳离子的SD大鼠起源的脂肪干细胞（ADSC）的生物安全性和神经诱导作用通过改进的多元醇方法合成的超顺磁性氧化铁（SPIO）纳米颗粒，以便可以使用体外MRI进行可视化。扫描方案以T2映射序列执行;同时，用透射电镜（TEM）观察标记细胞的超微结构，用电感耦合等离子体原子发射光谱法（ICP-AES）测量铁含量。神经诱导后，nestin和NSE（神经标记）明显表达。体外MRI显示，阳离子PEG / PEI改性的SPIO纳米粒子可实现出色的松弛性能和良好的寿命。 ICP-AES定量检测出的最低铁含量为1.56〜1.8μpg/ cell。这项研究表明，阳离子SPIO可以直接用于标记ADSC，然后可以诱导分化为神经并通过体外MRI成像，这对于进一步进行MRI对具有神经退行性疾病的动物模型具有重要的指导意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号