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首页> 外文期刊>Crop science >Protein Alterations in Tall Fescue in Response to Drought Stress and Abscisic Acid
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Protein Alterations in Tall Fescue in Response to Drought Stress and Abscisic Acid

机译：高羊茅对干旱胁迫和脱落酸的蛋白质变化

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Drought stress may alter protein synthesis in turfgrasses. The objectives of this study were to investigate physiological changes associated with the synthesis of dehydrin and a cytosolic-heat shock protein (HSC 70) in response to drought stress in two tall fescue (Festuca arundinacea L.) cultivars, a€?Southeasta€? and a€?Rebel Jr.a€?. The effects of abscisic acid (ABA) application on the drought tolerance of the cultivars also were evaluated. The cultivars were subjected to three treatments in growth chambers: well-watered control, drought stress, and drought stress following ABA treatment. Turf quality and leaf relative water content (RWC) decreased and electrolyte leakage (EL) increased during drought stress for both cultivars. The ABA-treated plants maintained higher turf quality and RWC, and lower EL than untreated plants under drought stress conditions. Levels of 20- and 29-kDa polypeptides increased during drought stress, and a 35-kDa polypeptide was noted in both cultivars only when subjected to drought stress either with or without ABA treatment. Immunoblot analysis indicated that dehydrin-like polypeptides of about 23-60 kDa were induced by progressive water deficit in both cultivars. The 53 kDa dehydrin polypeptide was present in Southeast with or without ABA treatment at 10 d of drought stress, whereas the 40 kDa dehydrin polypeptide accumulated in Rebel Jr. in both treatment. The 23- and 27-kDa dehydrin polypeptides were present at 10 d in drought-stressed and ABA-treated plants in both cultivars, but were more pronounced in the drought-stressed plants without ABA. A cytosolic-heat shock protein (HSC 70) was detected in plants in all treatments including well-watered plants of both cultivars, but its levels were higher in drought-stressed and ABA-treated plants. No single dehydrin polypeptide was induced by ABA treatment under drought stress, however, the promotive effects of ABA on the reduced drought stress paralleled the delayed induction of protein synthesis in tall fescue.
机译：干旱胁迫可能会改变草皮草中蛋白质的合成。这项研究的目的是调查在两个高羊茅（Festuca arundinacea L.）品种（东南）中干旱胁迫下与脱水素和胞质热休克蛋白（HSC 70）合成相关的生理变化。还有一个“叛军小”。还评估了脱落酸（ABA）对品种耐旱性的影响。栽培品种在生长室中进行了三种处理：控制良好的干旱，干旱胁迫和ABA处理后的干旱胁迫。在两个品种的干旱胁迫下，草皮质量和叶片相对含水量（RWC）降低，电解质泄漏（EL）增加。在干旱胁迫条件下，ABA处理的植物比未处理的植物保持更高的草皮质量和RWC，以及更低的EL。在干旱胁迫期间，20-和29-kDa多肽的水平增加，并且仅在经受或不使用ABA处理的干旱胁迫下，两个品种中才注意到35-kDa多肽。免疫印迹分析表明，两个品种中进行性缺水诱导了约23-60 kDa的脱水蛋白样多肽。在干旱胁迫10 d时，有或没有进行ABA处理的东南地区均存在53 kDa脱水素多肽，而两种处理中Rebel Jr.中均积累了40 kDa脱水素多肽。在两个品种的干旱胁迫和ABA处理植物中，在第10天都存在23 kDa和27 kDa脱水素多肽，但在没有ABA的干旱胁迫植物中更明显。在所有处理中，包括两个品种都浇水良好的植物中，均检测到了胞质热休克蛋白（HSC 70），但在干旱胁迫和ABA处理的植物中其含量较高。在干旱胁迫下，ABA处理没有诱导单个脱水蛋白多肽，但是，ABA对减轻干旱胁迫的促进作用与高羊茅中蛋白质合成的延迟诱导平行。

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