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Simple Sequence Repeat Diversity among Soybean Plant Introductions and Elite Genotypes

机译:大豆植物引种和优良基因型之间的简单序列重复多样性

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The use of molecular markers to facilitate the introgression of plant introduction (PI) germplasm into elite soybean [Glycine max (L.) Merr.] cultivars will depend on the amount of polymorphism that exists between elite genotypes and PIs. The objective of this study was to assess the simple sequence repeat (SSR) diversity of 39 elite soybean genotypes (Elites) and 40 PIs that were selected for high yield potential. A total of 397 alleles were detected among the 79 genotypes at 74 SSR marker loci. The number of alleles detected among the PIs was 30% greater than that detected among the Elites. There were 138 alleles specific to the PIs that occurred across 60 SSR loci and 32 alleles specific to the Elites that occurred across 27 SSR loci. Average marker diversity among the PIs was 0.56 and ranged from 0.0 to 0.84. Average marker diversity among the Elites was 0.50 and ranged from 0.0 to 0.79. Genetic similarity estimates based on simple matching coefficients revealed more genetic diversity among the PIs than among the Elites. The greatest genetic diversity was between the PIs and Elites. The ability of SSRs to distinguish among elite soybean genotypes and PIs with agronomic merit may assist with the transfer of favorable alleles from PIs into elite soybean cultivars.
机译:使用分子标记来促进植物导入(PI)种质向优良大豆[Glycine max(L.)Merr。]品种的渗入将取决于优良基因型和PI之间存在的多态性数量。这项研究的目的是评估39个优良大豆基因型(Elites)和40个PI的简单序列重复(SSR)多样性,这些品种被选作高产潜力。在74个SSR标记基因座的79个基因型中共检测到397个等位基因。在PI中检测到的等位基因数量比在精英中检测到的等位基因数量多30%。在60个SSR基因座中有138个特定于PI的等位基因,在27个SSR基因座中有32个特定于Elites的等位基因。 PI之间的平均标记多样性为0.56,范围从0.0到0.84。精英运动员的平均标记多样性为0.50,范围从0.0到0.79。基于简单匹配系数的遗传相似性估计显示,PI之间的遗传多样性要比精英组更高。最大的遗传多样性是在PI和精英之间。 SSRs区分具有优良农艺优势的优良大豆基因型和PI的能力可能有助于将有利的等位基因从PI转移到优良大豆品种中。

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