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首页> 外文期刊>Acta histochemica et cytochemica. >Localization of Rod Bipolar Cells in the Mammalian Retina Using an Antibody Against the α1c L-type Ca2+ Channel
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Localization of Rod Bipolar Cells in the Mammalian Retina Using an Antibody Against the α1c L-type Ca2+ Channel

机译：使用α 1 c L型Ca 2 + 通道的抗体在哺乳动物视网膜中的杆状双极细胞定位

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Bipolar cells transmit stimuli via graded changes in membrane potential and neurotransmitter release is modulated by Ca2+ influx through L-type Ca2+ channels. The purpose of this study was to determine whether the α 1c subunit of L-type voltage-gated Ca2+ channel ( α 1c L-type Ca2+ channel) colocalizes with protein kinase C alpha (PKC- α ), which labels rod bipolar cells. Retinal whole mounts and vertical sections from mouse, hamster, rabbit, and dog were immunolabeled with antibodies against PKC- α and α 1c L-type Ca2+ channel, using fluorescein isothiocyanate (FITC) and Cy5 as visualizing agents. PKC- α -immunoreactive cells were morphologically identical to rod bipolar cells as previously reported. Their cell bodies were located within the inner nuclear layer, dendritic processes branched into the outer plexiform layer, and axons extended into the inner plexiform layer. Immunostaining showed that α 1c L-type Ca2+ channel colocalized with PKC- α in rod bipolar cells. The identical expression of PKC- α and α 1c L-type Ca2+ channel indicates that the α 1c L-type Ca2+ channel has a specific role in rod bipolar cells, and the antibody against the α 1c L-type Ca2+ channel may be a useful marker for studying the distribution of rod bipolar cells in mouse, hamster, rabbit, and dog retinas.
机译：双极细胞通过膜电位的梯度变化传递刺激，并且通过L型Ca 2 + 通道的Ca 2 + 内流调节神经递质的释放。本研究的目的是确定L型电压门控Ca 2 + 通道的α 1 c亚基（α 1 c L型Ca 2 + 通道）与蛋白激酶Cα（PKC-α）共定位，该蛋白激酶标记杆状双极细胞。使用抗PKC-α和α 1 c L型Ca 2 + 通道的抗体对小鼠，仓鼠，兔和狗的视网膜整座和垂直切片进行免疫标记异硫氰酸荧光素（FITC）和Cy5作为显像剂。如先前报道，PKC-α-免疫反应性细胞在形态上与杆状双极细胞相同。它们的细胞体位于内核层内，树突突入外丛状层，而轴突则伸入内丛状层。免疫染色显示杆状双极细胞中α 1 c L型Ca 2 + 通道与PKC-α共定位。 PKC-α和α 1 c L型Ca 2 + 通道的相同表达表示α 1 c L型Ca < sup> 2 + 通道在杆状双极细胞中具有特定作用，针对α 1 c L型Ca 2 + 通道的抗体可能是一种用于研究杆状双极细胞在小鼠，仓鼠，兔和狗视网膜中的分布的有用标记。

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