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首页> 外文期刊>Czech Journal of Animal Science >Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kinetics
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Effect of protein secondary structures in mixed feedstuff detected by Fourier transform infrared spectroscopy on ruminal protein degradation kinetics

机译：傅立叶变换红外光谱法检测混合饲料中蛋白质二级结构对瘤胃蛋白质降解动力学的影响

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The objective of this study was to investigate the relationship between the protein secondary structure and the protein rumen degradation kinetics and the protein fractions of mixed feedstuffs of soybean meal with distillers dried grains with solubles (DDGS) at five mixed ratios (DDGS to soybean meal: 100?:?0, 70?:?30, 50?:?50, 30?:?70, 0?:?100). The Fourier transform infrared (FTIR) as a novel and cheap approach was used to detect the protein secondary structure, and the in situ nylon bag method was used to measure degradation kinetics of protein. Protein fractions were classified based on the Cornell net carbohydrate protein system. The results showed that (1) with the increasing soybean meal rate, the ruminal degraded protein and fractions of PB1 and PB2 were changed, (2) a higher α-helix to β-sheet ratio could result in a higher ruminally degraded protein, lower PB3 and PC, and higher PB1 and PB2 fractions in the feedstuff. So, mixing processing changed the feedstuff protein molecular structure spectral feature, which could influence the rumen degradation kinetics and protein fractions. It was inferred that protein degradation rate in mixed feedstuff can be measured by FTIR.
机译：这项研究的目的是研究五级混合比例（DDGS与豆粕：DDGS与豆粕的混合物）与豆粕混合饲料的蛋白质二级结构与蛋白质瘤胃降解动力学和蛋白质级分之间的关​​系： 100？：？0，70？：？30，50？：？50，30？：？70，0？：？100）。傅里叶变换红外光谱（FTIR）是一种新颖且便宜的方法，用于检测蛋白质的二级结构，而原位尼龙袋法用于测量蛋白质的降解动力学。基于康奈尔净碳水化合物蛋白质系统对蛋白质级分进行分类。结果表明：（1）随着豆粕率的增加，瘤胃降解蛋白质以及PB1和PB2的比例发生变化;（2）较高的α-螺旋与β-片层之比可能导致较高的瘤胃降解蛋白质，降低饲料中的PB3和PC，以及更高的PB1和PB2馏分。因此，混合加工改变了饲料蛋白质分子结构的光谱特征，这可能影响瘤胃降解动力学和蛋白质组分。可以通过FTIR测定混合饲料中的蛋白质降解率。

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