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首页> 外文期刊>Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis >Fungi of the iFusarium/i genus in the grains of conventional hybrids and transgenic Bt-hybrids of maize (iZea mays/i L.) in the Czech Republic
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Fungi of the iFusarium/i genus in the grains of conventional hybrids and transgenic Bt-hybrids of maize (iZea mays/i L.) in the Czech Republic

机译：捷克共和国玉米的常规杂种和转基因Bt-杂种谷物中 Fusarium 属的真菌

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Fungi of the iFusarium /igenus, the agent of ear rot in maize, not only causes decrease in yields but also negatively affects grain quality and, in relation to mycotoxins production, the health of humans and animals. This study focuses on determining the species range of iFusarium/i fungi in naturally infected stands of conventional hybrids and transgenic Bt-hybrids of maize in the Czech Republic during 2008 and 2009. Individual species of the iFusarium/i genus were determined on the basis of morphological characteristics and using polymerase chain reaction. Ten mycotoxigenic species were identified in hybrid maize grains: iF. subglutinans/i (40.4%), iF. graminearum/i (19.8%), iF. verticillioides/i (18.2%)i, F. poae /i(9.3%), iF. proliferatum /i(4.0%),i F. avenaceum/i (3.8%), iF. oxysporum/i (1.7%),i F. sporotrichioides/i (1.3%), iF. sambucinum/i (1.3%) and iF. culmorum/i (0.2%). The species iF. subglutinans/i, iF. graminearum/i and iF. verticillioides/i were dominant in both years. The frequency of individual iFusarium/i species did not significantly differ between conventional and transgenic Bt-hybrids. Differences in species representation were determined between individual years and sites. The hypothesized markedly lower infection of individual Bt-hybrids with fungi of the iFusarium/i genus was not statistically significant, although most Bt-hybrids did demonstrate lower infection without that being statistically significant. The average level of infection by the iFusarium/i genus in 2008 was 13.2% for grains of conventional hybrids and 6.6% for Bt-hybrids (50% lower). In 2009, the average infection level was 13.6% for conventional hybrids and 12.6% for Bt-hybrids (7.4% lower). The average infection level for grains of Bt-hybrids by the species iF. subglutinans/i, iF. graminearum/i and iF. proliferatum/i was lower than that for grains of conventional hybrids in both years.
机译：镰刀菌属真菌，是玉米中的玉米穗腐病病菌，不仅导致产量下降，而且对谷物品质产生不利影响，并且与霉菌毒素的生产有关，对人类和动物的健康也有不利影响。这项研究的重点是确定2008年至2009年捷克共和国传统杂交种和转基因Bt玉米杂交种自然感染的 Fusarium 真菌的物种范围。 Fusarium <根据形态特征并使用聚合酶链反应确定属。在杂种玉米籽粒中鉴定出十种致毒物种：F。次谷蛋白（40.4％）， F。禾本科（19.8％）， F。丝状葡萄（18.2％），细角拟南芥（9.3％）， F。 proliferatum （4.0％）， avenaceum （3.8％）， F。尖孢子虫（1.7％），孢子孢子虫（1.3％），F.桑布霉素（1.3％）和 F。 cul （0.2％）。物种 F。次谷蛋白， F。禾本科和 F。在这两年中，类黄铁质占主导地位。传统的和转基因的Bt杂种之间，各个 Fusarium 种类的频率没有显着差异。确定了不同年份和地点之间物种代表的差异。尽管大多数Bt杂种确实显示出较低的感染率，但统计学上无统计学意义，但假想的Ftarium 属真菌对各个Bt杂种的感染率均没有显着统计学意义。 2008年， Fusarium 属的平均感染水平是常规杂种的13.2％和Bt杂种的6.6％（低50％）。 2009年，传统杂种的平均感染水平为13.6％，Bt杂种的平均感染水平为12.6％（降低7.4％）。 Ft物种对Bt-杂种谷物的平均感染水平。次谷蛋白， F。禾本科和 F。两年中，proliferatum 均低于常规杂种的谷粒。

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