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首页> 外文期刊>Acta veterinaria scandinavica >Validation of an enzyme-linked immunosorbent assay developed for measuring cortisol concentration in human saliva and serum for its applicability to analyze cortisol in pig saliva
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Validation of an enzyme-linked immunosorbent assay developed for measuring cortisol concentration in human saliva and serum for its applicability to analyze cortisol in pig saliva

机译：用于测定人唾液和血清中皮质醇浓度的酶联免疫吸附测定法的验证性，以用于分析猪唾液中的皮质醇

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Background The purpose of this study was to validate a commercially available enzyme-linked immunosorbent assay (ELISA) developed for measuring free cortisol in human saliva and total cortisol concentration in diluted human serum, for its applicability in measuring cortisol concentration in pig saliva. Collection of saliva is less stressful than e.g. blood sampling, and is a non-invasive method. Findings Saliva was collected by allowing sows to chew on cotton swabs held by forceps. Thereafter, the swabs were centrifuged to retrieve the saliva. The ELISA was performed according to instructions provided by the manufacturer. To validate the ELISA, determination of the intra-assay coefficient of variation (CV), inter-assay CV, recovery, linearity and parallelism was performed. The intra-assay CV was below 10% and inter-assay CV below 15% for samples of high, medium and low cortisol concentrations. The mean recovery was 117% and the linearity and parallelism showed an r2-value of 0.994 and 0.993, respectively. For biological assessment of induced social stress, two saliva samples were collected in the morning from 6 primiparous and 21 multiparous sows. One sample was collected when the sows were individually housed in a farrowing pen and a second sample was collected when the sows were group housed. The primiparous sows had a significant higher cortisol concentration compared to the multiparous sows when group housed. Conclusion The results obtained in this validation study indicate that the ELISA is suitable for measuring cortisol concentration in porcine saliva.
机译：背景技术这项研究的目的是验证一种可用于测量人唾液中游离皮质醇和稀释人血清中总皮质醇浓度的商业化酶联免疫吸附测定（ELISA）方法，该方法可用于测量猪唾液中的皮质醇浓度。唾液收集的压力小于例如采血，是一种非侵入性方法。结果通过允许母猪咀嚼由镊子夹持的棉签来收集唾液。之后，将拭子离心以回收唾液。 ELISA根据制造商提供的说明书进行。为了验证ELISA，进行了测定内变异系数（CV），测定间变异系数，回收率，线性和平行度的测定。对于高，中和低皮质醇浓度的样品，测定内CV低于10％，测定间CV低于15％。平均回收率为117％，线性和平行度的r 2 值分别为0.994和0.993。为了对诱发的社会压力进行生物学评估，早晨从6头初产和21头多胎母猪中收集了两个唾液样本。当将母猪单独放在分娩猪舍中时收集一个样品，将母猪分组饲养时收集第二个样品。与群养母猪相比，初产母猪的皮质醇浓度明显更高。结论这项验证研究获得的结果表明ELISA适用于测定猪唾液中的皮质醇浓度。

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