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首页> 外文期刊>Advances in Bioscience and Biotechnology >Accumulation of amino acids deriving from pyruvate in Escherichia coli W3110 during fed-batch cultivation in a two-compartment scale-down bioreactor
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Accumulation of amino acids deriving from pyruvate in Escherichia coli W3110 during fed-batch cultivation in a two-compartment scale-down bioreactor

机译：在两室按比例缩小的生物反应器中分批补料培养过程中大肠杆菌W3110中丙酮酸衍生氨基酸的积累

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Bioprocess scale-down simulators are used to investigate the phenomena occurring in industrial scale cultivations. The aim is to simulate the large-scale conditions, characterized by long mixing times introducing local gradients into the bioreactor, in laboratory scale bioreactor. We have expanded the current physiological knowledge about the consequences of such cultivation conditions from well-known mixed-acid fermentation to amino acid metabolism. Several interesting deviations were observed for amino acids derived from pyruvate when a STR-PFR scale-down simulator and a regular STR fed-batch cultivation were compared. In this article we show that the cultivation of Escherichia coli K-12 substrain W3110 in a scale-down two-compartment bioreactor has consequences on the free amino acid pools. Repeated occurrence of high glucose zones affects the biosynthesis of amino acids derived from pyruvate. This is interesting since the pyruvate based amino acids are precursors of the branched chain amino acid pathway, which is further disturbed by an unbalanced regulation caused by a frameshift mutation in the ilvG gene of E. coli K-12 strains. As a consequence the formation of non-typical amino acid norvaline was detected in the two compartment bioreactor cultivation.
机译：生物过程按比例缩小模拟器用于研究工业规模种植中发生的现象。目的是在实验室规模的生物反应器中模拟大规模条件，其特征在于较长的混合时间将局部梯度引入生物反应器中。从已知的混合酸发酵到氨基酸代谢，我们已经扩大了有关这种培养条件后果的当前生理学知识。当比较STR-PFR比例缩小模拟器和常规STR补料分批培养时，对于丙酮酸衍生的氨基酸观察到一些有趣的偏差。在本文中，我们表明在按比例缩小的两室生物反应器中培养大肠杆菌K-12菌株W3110会对游离氨基酸池产生影响。高糖区的反复出现影响了丙酮酸衍生氨基酸的生物合成。这是令人感兴趣的，因为基于丙酮酸的氨基酸是支链氨基酸途径的前体，其进一步被大肠杆菌K-12菌株的ilvG基因的移码突变引起的不平衡调节所干扰。结果，在两室生物反应器培养物中检测到非典型氨基酸正缬氨酸的形成。

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