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首页> 外文期刊>Advances in Life Sciences >Isolated of Bacillus Cereus in Chicken Meat and Investigation β-Lactamase Antibiotic-Resistant in Bacillus Cereus from Chicken Meat
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Isolated of Bacillus Cereus in Chicken Meat and Investigation β-Lactamase Antibiotic-Resistant in Bacillus Cereus from Chicken Meat

机译：鸡肉中蜡状芽孢杆菌的分离及对鸡肉中蜡状芽孢杆菌的β-内酰胺酶抗药性研究

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Background : Bacillus cereus is an opportunistic pathogen one of the major causing food poisoning, manifested by diarrhea or emetic syndrome. The present study undertaken to isolation B. cereus by Phenotype & Genotype; characterization of β-lactamase from B. cereus strain isolated from raw chicken meat. B. cereus produces a broad-spectrum β-lactamase and it is one of the potential Pathogenic reasons that makes the strains resistant, the blm β-lactamase is a gene that has the ability to resist. Materials and Methods : A total 250 sample collected from Breast, Tight, Wing, Liver and Gizzard of Raw Chicken from Sanandaj of Iran by PEMPA selective media, 39 B. cereus Isolated by 7, β-lactamse discs examined by antibiogram method, DNA extraction by CTAB and, the resistance strain checked for blm β-lactamse Resistance Gene by PCR. Result: The isolates of B. Cereus (15.6%) from chicken meat were highest resistance to Penicillin, Cloxacilin, Cephalexin (100%) and the least in Ampicillin (13%). 76.4% of the isolated Bacillus cereus has been blm. Discussion: This study examines the prevalence of B. cereus in chicken meat. The estimate that the poultry slaughterhouse contaminated with Bacillus cereus and will find its presence in the environment, can induce illness, The accessibility of appropriate protocols for treatment with β- lactamase antibiotics that comprise the bulk of antibiotics to use in clinics, poultry flocks and veterinary.
机译：背景：蜡状芽孢杆菌是一种机会病原体，是引起食物中毒的主要原因之一，表现为腹泻或呕吐综合症。本研究旨在通过表型和基因型分离蜡状芽孢杆菌。鸡肉中分离的蜡状芽孢杆菌β-内酰胺酶的特性蜡状芽孢杆菌产生广谱的β-内酰胺酶，这是使菌株具有抗性的潜在病原性原因之一，blmβ-内酰胺酶是具有抗性能力的基因。 材料和方法：通过PEMPA选择性培养基从伊朗Sanandaj的生鸡肉的乳房，紧身，翅膀，肝脏和G中收集了总共250个样品，蜡状芽孢杆菌通过7，β-分离出蜡状芽孢杆菌。通过抗菌素法检查内酰胺盘，通过CTAB提取DNA，并通过PCR检查抗性菌株的blmβ-内酰胺酶抗性基因。结果：从鸡肉中分离出的蜡状芽孢杆菌（15.6％）对青霉素，克洛西林，头孢氨苄的抵抗力最高（100％），而对氨苄西林的抵抗力最低（13％）。分离的蜡状芽孢杆菌中有76.4％是blm。讨论：这项研究检查了蜡状芽孢杆菌在鸡肉中的流行情况。估计家禽屠宰场被蜡样芽孢杆菌污染，并会在环境中发现，会诱发疾病。使用β-内酰胺酶抗生素治疗的适当方案的可及性包括临床，家禽和兽医中使用的大量抗生素。

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