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首页> 外文期刊>Advances in Life Sciences >Production of Protease and Amylase from Bacillus subtilis and Aspergillus niger Using Parkia biglobossa (Africa Locust Beans) as Substrate in Solid State Fermentation
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Production of Protease and Amylase from Bacillus subtilis and Aspergillus niger Using Parkia biglobossa (Africa Locust Beans) as Substrate in Solid State Fermentation

机译：由芽孢杆菌 s ubtilis 和曲霉 n n 产生蛋白酶和淀粉酶iger 在固态发酵中使用 Parkia b iglobossa （非洲刺槐豆）作为底物

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Bacillus subtilis and Aspergillus niger were utilized for the production of amylase and protease enzymes in this study. Parkia biglobossa (Africa Locust Beans) shell was used as substrate by both organisms for the production of amylase and protease enzyme. The optimum temperature for the activity of amylase and protease enzymes produced by Bacillus subtilis was 50~(o)C, while 30~(o)C and 40~(o)C was recorded for amylase and protease enzyme produced by Aspergillus niger with an activity of 1.1 mg/ml/sec, 0.8 mg/ml/sec for amylase and protease enzyme by Bacillus subtilis and 0.87 mg/ml/sec, 0.77 mg/ml/sec for amylase and protease produced by Aspergillus niger respectively. Optimum pH was attained at pH 9 for amylase and protease enzyme produced by Bacillus subtilis with an activity of 1.2 mg/ml/sec and 0.83 mg/ml/sec respectively. The optimum pH for the activity of Aspergillus niger was recorded at pH 5 and pH 6 for amylase and protease with an activity of 0.87 mg/ml/sec and 0.74mg/ml/sec respectively. The result showed that both organisms utilized Parkia biglobossa to produce extracellular amylase and protease, but the activity of amylase enzyme produced by both organisms was greater than the activity of protease enzyme and enzymes produced by Bacillus subtilis showed superior activity which can be useful industrially.
机译：在本研究中，枯草芽孢杆菌和黑曲霉被用于生产淀粉酶和蛋白酶。两种生物都使用帕克大球果（非洲刺槐豆）壳作为底物来生产淀粉酶和蛋白酶。枯草芽孢杆菌产生的淀粉酶和蛋白酶活性的最适温度为50〜（o）C，记录的枯草芽孢杆菌产生的淀粉酶和蛋白酶的最适温度为30〜（o）C和40〜（o）C。 i>黑曲霉，对枯草芽孢杆菌的淀粉酶和蛋白酶的活性为1.1 mg / ml / sec，0.8 mg / ml / sec，对淀粉酶和蛋白酶的活性为0.87 mg / ml / sec，0.77 mg / ml / sec分别由黑曲霉（Aspergillus niger）生产。由枯草芽孢杆菌产生的淀粉酶和蛋白酶的最适pH为9，活性分别为1.2mg / ml / sec和0.83mg / ml / sec。记录黑曲霉活性的最佳pH在淀粉酶和蛋白酶的pH 5和pH 6下，其活性分别为0.87mg / ml / sec和0.74mg / ml / sec。结果表明，两种生物均利用大果Parkia biglobossa产生细胞外淀粉酶和蛋白酶，但两种生物产生的淀粉酶的活性均大于蛋白酶的活性，枯草芽孢杆菌产生的酶表现出优异的活性。在工业上是有用的。

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