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首页> 外文期刊>Advances in Environmental Technology >Characterization and Kinetic study of PAH–degrading Sphingopyxis ummariensis bacteria isolated from a petrochemical wastewater treatment plant
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Characterization and Kinetic study of PAH–degrading Sphingopyxis ummariensis bacteria isolated from a petrochemical wastewater treatment plant

机译：石油化工废水处理厂中降解多环芳烃的Sphingopyxis ummariensis细菌的表征和动力学研究

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The expansion of a microbial bank for the degradation of polycyclic aromatic hydrocarbons (PAHs) is crucial for removal of these persistent pollutants. In this study, five gram-negative, aerobic, non-fermentative bacterial strains (III-R3, IV-P11, IV-P13, IV-R13, and V-P18) were isolated from the activated sludge of a petrochemical wastewater treatment plant using enrichment pro tocol based on phenanthrene. The isolates were capable of utilizing phenanthrene, anthracene, and pyrene as a sole carbon and energy source in an aerobic batch aqueous system. The PAHs biodegradation yields were evaluated by gas chromatography and the bacterial isolates were identified using the 16S rRNA sequencing method. A first-order kinetic model provided the best fit to the phenanthrene degradation profiles with a correlation coefficient value of 0.95-0.98. The phenanthrene biodegradation rate constants and half-lives were measured at the range 0.653--Q.878 day-' and 0.79-1.06 day, respectively. Lower values of Anthracene degradation re sulted with the isolates of the current study, while a relatively high percentage of the removal of Pyrene was obtained by some of the isolates. The data obtained in this study shows that bacterial isolates have degradation preference over Mycobacterium sp. and Pseudomonas aeruginosa; and they are comparable with Pseudomonas stutzeri, Sphingomonas sp., and microbial consortium applied by other researchers. Analysis of the 16S rDNA gene sequence, when compared with the GenBank, indicates that all the strains belong to the genus Sphingopyxis with the nearest type strain being Sphingopyxis ummariensis Ul2 (MTCC 8591T). It is the first time that Sphingopyxis ummariensis is reported for its capability in the degradation of PAHs.
机译：扩展微生物库以降解多环芳烃（PAH）对于清除这些持久性污染物至关重要。在这项研究中，从石化废水处理厂的活性污泥中分离出五种革兰氏阴性，需氧，非发酵细菌菌株（III-R3，IV-P11，IV-P13，IV-R13和V-P18）。使用基于菲的富集协议。分离株能够利用菲，蒽和pyr作为好氧分批水性系统中唯一的碳和能源。通过气相色谱法评估PAHs的生物降解率，并使用16S rRNA测序方法鉴定细菌分离株。一阶动力学模型以0.95-0.98的相关系数值提供了与菲降解曲线的最佳拟合。菲的生物降解速率常数和半衰期分别在0.653-Q.878天-和0.79-1.06天的范围内测量。当前研究的分离物导致蒽降解值较低，而某些分离物获得的high去除率相对较高。在这项研究中获得的数据表明，细菌分离株比分枝杆菌具有降解优先权。和铜绿假单胞菌;它们可与斯图氏假单胞菌，鞘氨醇单胞菌和其他研究人员应用的微生物财团媲美。与GenBank相比，对16S rDNA基因序列的分析表明，所有菌株均属于Sphingopyxis属，最近型菌株为Sphingopyxis ummariensis Ul2（MTCC 8591T）。这是第一次报道了狮身鱼蛛（Sphingopyxis ummariensis）具有降解PAHs的能力。

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