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首页> 外文期刊>ACS Omega >Investigating the Catalytic Activity of Glycosyltransferase on Quercetin from Tripterygium wilfordii
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Investigating the Catalytic Activity of Glycosyltransferase on Quercetin from Tripterygium wilfordii

机译：研究糖基转移酶对雷公藤槲皮素的催化活性

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Flavonoid glycosides have shown many pharmacological activities in clinical studies. However, the main way to obtain flavonoid glycosides is to extract and separate them from plants, which wastes both time and resources. Here, we identified the O-glycosyltransferase (UGTs) TwUGT3 from Tripterygium wilfordii and analyzed its bioinformatics. First, the enzyme was found to utilize phloretin and uridine diphosphate glucose (UDPG) as substrates to produce an acid-tolerant glucoside. Then, it also can use quercetin and UDPG as substrates to produce the corresponding O-glucoside. In addition, we further explored the substrate specificity of TwUGT3, which suggested that it also accepts luteolin, pinocembrin, and genistein to produce the corresponding O-glucosides. Subsequently, the optimum pH, reaction time, reaction temperature, and enzymatic kinetic parameters of TwUGT3 were determined.
机译：黄酮苷在临床研究中已显示出许多药理活性。然而，获得类黄酮糖苷的主要方法是将其从植物中提取并分离出来，这浪费了时间和资源。在这里，我们鉴定了雷公藤的O-糖基转移酶（UGTs）TwUGT3，并分析了其生物信息学。首先，发现该酶利用促视紫红质和尿苷二磷酸葡萄糖（UDPG）作为底物来产生耐酸的葡萄糖苷。然后，它也可以使用槲皮素和UDPG作为底物来生产相应的O-葡萄糖苷。此外，我们进一步探索了TwUGT3的底物特异性，这表明它也接受木犀草素，松皮精和金雀异黄素来产生相应的O-葡萄糖苷。随后，确定了TwUGT3的最佳pH，反应时间，反应温度和酶动力学参数。

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