...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>ACS Omega >pH-Regulated Synthesis of Trypsin-Templated Copper Nanoclusters with Blue and Yellow Fluorescent Emission
【24h】

pH-Regulated Synthesis of Trypsin-Templated Copper Nanoclusters with Blue and Yellow Fluorescent Emission

机译：pH值调节的蓝色和黄色荧光发射的胰蛋白酶模板的铜纳米簇的合成。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this article, a simple protocol to prepare water-soluble fluorescent copper nanoclusters (CuNCs) using trypsin as a stabilizer and hydrazine hydrate as a reducing agent was reported. It was found that the pH of the reaction solution was critical in determining the fluorescence of CuNCs. CuNCs with blue and yellow fluorescent emission were obtained under basic and acidic conditions, respectively. Although the detailed formation mechanisms of these CuNCs required further analysis, the synthetic route was promising for preparing different fluorescent metal NCs for applications. With good water solubility and excellent photostability, the yellow-emitting CuNCs could serve as a fluorescence probe for detection of Hg~(2+) based on the aggregation-induced quenching mechanism. The fluorescence quenching efficiency had fantastic linearity to Hg~(2+) concentrations in the range of 0.1–100 μM, with a limit of detection of 30 nM. Additionally, the yellow-emitting CuNCs exhibited negligible cytotoxicity and were successfully applied to bioimaging of HeLa cells.
机译：在本文中，报告了使用胰蛋白酶作为稳定剂和水合肼作为还原剂制备水溶性荧光铜纳米簇（CuNC）的简单协议。已经发现，反应溶液的pH对于确定CuNCs的荧光至关重要。分别在碱性和酸性条件下获得具有蓝色和黄色荧光发射的CuNC。尽管这些CuNCs的详细形成机理需要进一步分析，但合成路线有望用于制备不同的荧光金属NCs。发射黄色的CuNCs具有良好的水溶性和优异的光稳定性，可根据聚集诱导的猝灭机理，用作检测Hg〜（2+）的荧光探针。荧光猝灭效率与Hg〜（2+）浓度在0.1–100μM范围内具有极好的线性关系，检出限为30 nM。此外，发黄光的CuNCs的细胞毒性微不足道，已成功应用于HeLa细胞的生物成像。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号