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首页> 外文期刊>Advances in Forestry Science >Organogenesis in zygotic embryos and hypocotyls of physic nut (Jatropha curcas L.) under 6-benzylaminopurine levels
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Organogenesis in zygotic embryos and hypocotyls of physic nut (Jatropha curcas L.) under 6-benzylaminopurine levels

机译：6-苄基氨基嘌呤水平下合子胚和物理坚果（麻疯树）下胚轴的器官发生

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摘要

Physic nut had a great potential for biodiesel production and presents higher oil content. The aim of this study was obtain organogenesis in zygotic embryos and hypocotyls of physic nut using 6-benzylaminopurine (BAP). Seeds were disinfected and after then cotyledons were removed; they were in vitro germinated at 0, 1, 2, 3, 4, 5 and 6 mg.L -1 BAP. Hypocotyls were excised from germinated seeds with 45 days old and cultured at 0, 1, 2.5 and 5 mg.L -1 BAP. Callus from zygotic embryos were cultured at double-phase medium being liquid medium supplemented with 1, 2.5 and 5 mg.L -1 BAP. The largest percentage of callus formation in zygotic embryos occurred at 6 mg.L -1 BAP. On the other hand, to induce shoot from zygotic embryos the best BAP level was 4 mg.L -1 . Callogenesis in hypocotyls can be efficiently induced with 1 mg.L -1 BAP. Double-phase medium results in lowest protuberances rate, varying from 0 to 3 per callus. In order to induce callus from zygotic embryos and hypocotyls of physic nut, BAP is sustainable.
机译：物理坚果具有生产生物柴油的巨大潜力，并具有较高的油含量。这项研究的目的是使用6-苄基氨基嘌呤（BAP）获得合子胚和物理坚果的下胚轴的器官发生。将种子消毒，然后将子叶去除;它们在体外以0、1、2、3、4、5和6 mg.L -1 BAP萌发。从45天大的发芽种子中切下胚轴，并以0、1、2.5和5mg.L -1 BAP培养。将合子胚的愈伤组织在补充有1、2.5和5mg.L -1 BAP的液体培养基的双相培养基中培养。合子胚中愈伤组织形成的最大百分比发生在6 mg.L -1 BAP。另一方面，要诱导合子胚芽，最佳BAP水平为4 mg.L -1。 1 mg.L -1 BAP可以有效地诱导下胚轴的发生。双相培养基导致最低的突出率，每个愈伤组织从0到3不等。为了从合子胚和物理坚果的下胚轴诱导愈伤组织，BAP是可持续的。

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